评估和量化复杂生物系统中氧化应激生物标志物的战略方法
Padmasri Sai Nandana Aravapally1, Naveen Chandrasekar1, Arvind Verma1
1Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research - Ahmedabad (NIPER-A), Opposite Air force Station Palaj, Gandhinagar, India.
Bioanalysis
|April 4, 2025
概括
氧化应激 (OS) 监测对于疾病诊断和进展至关重要. 本综述详细介绍了评估OS生物标志物的分析技术,这对于理解和管理OS相关条件至关重要.
科学领域:
- 生物医学科学 生物医学科学
- 临床研究 临床研究
- 分析化学 分析化学
背景情况:
- 氧化应激 (OS) 产生于反应性氧物种 (ROS) 生产和细胞修复机制之间的不平衡.
- 慢性OS与主要疾病有关,包括糖尿病,心血管疾病,癌症和神经退行性疾病.
- 准确的OS监测对于疾病诊断,进展评估和临床结果预测至关重要.
研究的目的:
- 审查和评估用于评估氧化应激生物标志物的分析技术.
- 探索用于OS生物标志物检测的样品制备和分析方法的进展.
- 提供对氧化应激生物标志物分析技术的全面评估.
主要方法:
- 使用谷歌学者,PubMed和Reaxys进行全面的文献搜索.
- 针对氧化应激生物标志物和分析技术的研究的识别和分析.
- 在复杂的生物矩阵中基于灵敏度,特异性和可靠性的技术的评估.
主要成果:
- 开发有效的分析技术,用于评估生物流体中的OS生物标志物.
- 样品准备程序的进步提高了生物标志物的检测.
- 介绍了各种分析方法的详细规范和性能评估.
结论:
- 有效的分析技术可用于通过脂质过氧化,DNA损伤和蛋白质氧化等生物标志物间接评估氧化应激.
- 需要进一步探索分析方法,以应对低度生物标志物的敏感性和特异性的挑战.
- 本综述提供了对生物标志物分析的彻底评估,有助于理解和管理氧化应激.
相关概念视频
01:29Oxygen Requirements and Growth Patterns
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
01:30Methods to Assess Microbial Populations
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
01:19Methods to Assess Microbial Communities
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...