相关实验视频

Updated: Jun 12, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

鉴定了一种与帕金森病进展相关的circRNA-miRNA-mRNA相互作用体

Lisa Shrestha¹, André Leier^1,2

¹Department of Genetics, University of Alabama at Birmingham, School of Medicine, Birmingham, AL, USA.

Journal of Parkinson's disease

|April 4, 2025

概括

循环RNAs (circRNAs) 是帕金森病 (PD) 进展的关键指标. 这项研究确定了特定的circRNA及其点,将其与疾病进展和UPDRS III分数联系起来.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
神经科学是一个神经科学.

背景情况:

循环RNAs (circRNAs) 是稳定的RNA分子,参与细胞调节.
circRNAs被探索为疾病的生物标志物,包括神经退行性疾病,如帕金森病 (PD).
以前的研究已经确定了PD中的circRNAs,但没有一个专注于疾病进展.

研究的目的:

为了研究circRNAs在帕金森病 (PD) 进展中的作用.
确定与PD进展相关的circRNA及其下游调节网络.
确定与PD进展相关的潜在miRNA-mRNA目标.

主要方法:

对来自帕金森病进展标志物倡议 (PPMI) 和帕金森病生物标志物计划 (PDBP) 队列的大规模纵向RNA测序数据的分析.
随着时间的推移,在早期帕金森病 (PD) 患者中,circRNA表达的表征.
竞争性内源RNA (ceRNA) 网络的映射和共同表达分析以确定与PD相关的基因.

主要成果:

六个circRNA被发现在PD患者中随着时间的推移显著差异地表达.
确定了一个具有下游miRNA-mRNA目标的ceRNA网络.
环RNA表达和基因标的变化与UPDRS III分数有显著的相关性,表明PD进展.

结论:

循环RNAs (circRNAs) 与帕金森病 (PD) 的进展有显著的关联.
失调的circRNA表达作为PD进展的关键分子标记.
已识别的circRNA及其网络为PD病原和治疗策略提供了潜在的见解.

关键词:

帕金森病生物标志物计划帕金森病的进展和进展帕金森病进展标记者倡议在 UPDRS III (上) 部分.圆形的RNARNA是一个圆形的RNA.共同表达网络的共同表达网络.具有竞争的内源性RNA.分子生物标志物分子生物标志物稀疏的部分相关性在基因表达上重量基因相关性网络分析

更多相关视频

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Published on: March 21, 2020

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Neural Regulation

Neural Regulation

Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.

Parkinson's Disease: Overview

Parkinson's Disease: Overview

Neurodegenerative disorders are progressive diseases that cause irreversible damage and loss to neurons in specific brain areas. Examples of these disorders include Parkinson's disease, Alzheimer's disease, Multiple Sclerosis (MS), and Amyotrophic Lateral Sclerosis (ALS). These disorders share characteristics such as proteinopathies, selective neuronal vulnerability, and a complex interplay between genetic and environmental factors. The primary therapeutic goal for these conditions is...