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Updated: May 16, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

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西格马托普重新排列使得可以获得高度稳定的螺旋环亚氧化物,用于蛋白质旋转标签

Mateusz P Sowiński1, Elena M Mocanu2, Hannah Ruskin-Dodd2

  • 1Department of Chemistry, UiT The Arctic University of Norway, 9037 Tromsø, Norway. marius.haugland-grange@uit.no.

Chemical communications (Cambridge, England)
|April 4, 2025
PubMed
概括

研究人员为电子磁共振 (EPR) 研究开发了一种新的持久旋转标签. 这种探测器可以在低温下使用双电子共振 (DEER) 精确测量像calmodulin这样的生物分子中的距离.

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[3,3] Sigmatropic Rearrangement of 1,5-Dienes: Cope Rearrangement

The Cope rearrangement is classified as a [3,3] sigmatropic shift in 1,5-dienes, leading to a more stable, isomeric 1,5-diene. The reaction involves a concerted movement of six electrons, four from two π bonds and two from a σ bond, via an energetically favorable chair-like transition state.
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Sigmatropic rearrangements are a class of pericyclic reactions in which a σ bond migrates from one part of a π system to another. These are intramolecular rearrangements where the total number of σ and π bonds remain unchanged.
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Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
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