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在pH值转移的协同效应超声波中修改了Hericium erinaceus蛋白质的提取

Pin Gong^1,2,3, Ke Xu^1,2,3, Shan Yue^1,2,3

¹School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, China.

Journal of food science

|April 4, 2025

概括

这项研究优化了Hericium erinaceus蛋白 (HEP) 提取,使用性条件和超声波辅助的pH偏移处理. 最佳的条件可以增强HEP.

科学领域:

食品科学食品科学食品科学
生物技术是生物技术.
菌类学菌类学是指菌类学.

背景情况:

(Hericium erinaceus,HE) 是一种功能性,具有潜在的健康益处.
高效提取和修改HE蛋白 (HEP) 对于其应用至关重要.
现有的蛋白质提取方法可能缺乏效率或可持续性.

研究的目的:

为了优化Hericium erinaceus蛋白 (HEP) 的性提取条件.
为了研究超声波辅助pH转移治疗对HEP活性的影响.
为绿色和高效的真菌蛋白修饰技术奠定基础.

主要方法:

研究了pH值,物质液体比,提取时间和温度对HEP提取速率的影响.
采用响应表面方法来优化性提取参数.
应用超声波辅助的pH转移处理来修改HEP并评估其功能性质.

主要成果:

HEP提取速率随着pH值的变化而变化,随着物质-液体比率,时间和温度的增加而增加,直到达到平原.
确定最佳的性提取条件:66°C,pH值12.5,材料与液体比为1:25.
超声波辅助的pH转移治疗 (31分钟,40°C,30W/L) 显著改善了HEP的胆盐结合和胆固醇抑制活性.

结论:

建立了高效性提取HEP的最佳参数.
已证明,超声波辅助的pH变化治疗是提高HEP功能的一种有效方法.
为功能性真菌蛋白的工业应用提供了基础.

关键词:

在体外低脂性活动.改变pH值的变化超声波超声波是指超声波的使用.

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