基于生物标志物的方法来评估人类对食品中的暴露
C Kalisch1, M Reiter1, A Mally2
1Department of Toxicology, University of Würzburg, Versbacher Str. 9, 97078, Würzburg, Germany.
Archives of toxicology
|April 4, 2025
概括
尿中的N-[4-carboxy-4-(3-mercapto-1H-pyrrol-1-yl)-1-oxobutyl]-L-cysteinylglycine (GSH-BDA) 含量随着富含的饮食而增加,验证了它作为估计人类暴露的生物标志物. 吸烟也显著影响GSH-BDA水平.
科学领域:
- 环境健康科学 环境健康科学
- 毒理学 毒理学 毒理学
- 生物标志物发现发现
背景情况:
- 人类面临着持续暴露于肝毒性和致癌性食品污染物 furan.
- 目前的暴露估计依赖于食物含量,但不确定性需要生物标志物探索.
- N-[4-carboxy-4-(3-mercapto-1H-pyrrol-1-yl)-1-oxobutyl]-L-cysteinylglycine (GSH-BDA),一种谷氨 (GSH) 结合物,此前已被确定为大鼠的特定生物标志物.
研究的目的:
- 验证GSH-BDA作为人类志愿者对外源性暴露的可靠生物标志物.
- 为了监测尿中的代谢物分泌,以应对控制的低和高饮食.
- 评估GSH-BDA适用于将生物标志物水平转化为可能的每日素摄入量.
主要方法:
- 十名健康的志愿者在9天内连续食用了低,高和低饮食.
- 用稳定同位素稀释液体染色学-并联质谱法 (LC-MS/MS) 分析了尿中的 furan 代谢物.
- 尿液中的GSH-BDA分泌量被量化以估计可能的每日素摄入量.
主要成果:
- 在高饮食期间,尿液中的GSH-BDA分泌量显著增加,并在恢复低饮食后迅速减少.
- 非吸烟者的GSH-BDA水平 (0.051.20μg/kg bw/d) 的估计每日素摄入量与欧洲食品安全局 (EFSA) 的报告一致.
- 在吸烟者中观察到更高的GSH-BDA分泌量,这表明烟草烟雾是重要的源和混因素.
结论:
- 尿液GSH-BDA是一种适合和特定的生物标志物,用于评估人类外源性暴露.
- 通过GSH-BDA分析,可以估计每天可能摄入的,从而补充了传统的食物含量方法.
- 在使用GSH-BDA进行饮食暴露评估时,必须考虑吸烟状态,因为它对暴露的贡献.
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