相关实验视频

Updated: May 6, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

通过矩阵辅助激光脱电离-飞行质谱仪的离子化时间精确识别引起细菌病的乳球菌

Marhami Fahriani¹, Geoffrey W Coombs^1,2,3, Princy Shoby¹

¹School of Medical, Molecular and Forensic Sciences, Murdoch University, Murdoch, Western Australia, Australia.

Journal of medical microbiology

|April 4, 2025

概括

一个新的自定义MALDI生物型数据库准确地识别了Enterococcus lactis,此前被错误分类为Enterococcus faecium. 这种工具有助于临床实验室区分E. lactis和E. faecium,改善患者的诊断和护理.

科学领域:

微生物学微生物学
临床诊断临床诊断临床诊断
细菌的分类学细菌的分类学

背景情况:

菌 (Enterococcus faecium clade B) 已经被重新分类为乳腺菌 (Enterococcus lactis).
由于其重新分类和潜在的疾病关联,准确识别E. lactis至关重要.
目前用于识别E. lactis的分子方法复杂,需要更简单的诊断工具.

研究的目的:

开发一个 MALDI Biotyper 定制数据库,以快速准确地识别 Enterococcus lactis.
为了区分导致细菌病的E. lactis和与之密切相关的Enterococcus faecium.
为了解决在临床环境中错误地将E. lactis识别为E. faecium.

主要方法:

用全基因组测序来确认97个肠球菌分离物的物种身份.
使用29个E. lactis分离物构建了一个MALDI Biotyper内部数据库.
使用97个孤立数据库验证了定制数据库,并将结果与标准MBT Compass参考库进行了比较.

主要成果:

定制的MALDI生物型数据库正确识别了所有E. lactis和E. faecium分离物.
所有的E. lactis分离物被标准商业数据库 (MBT Compass 2022) 错误地识别为E. faecium.
使用定制数据库,E. lactis和E. faecium的高 (≥2,300) 或可能 (2,000-2,299) 的物种级识别得分得到了实现.

结论:

一个经过验证的定制MALDI光谱数据库可以准确识别乳腺肠球菌.
这种工具可以很容易地在临床和研究实验室中实施.
它解决了标准商业平台目前错误地将E. lactis识别为E. faecium.

关键词:

肠球菌 (Enterococcus faecium) 是一个有毒的菌株.乳腺肠球菌 (Enterococcus lactis) 是一个细菌.马尔迪-托夫 (MALDI-TOF) 是一个国家元首.细菌血症的发生

更多相关视频

Field Identification of Matricaria chamomilla using a Portable qPCR System

Field Identification of Matricaria chamomilla using a Portable qPCR System

Published on: October 10, 2020

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Published on: May 24, 2024

相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...