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Updated: May 17, 2025

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
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来自机会性病原体Acinetobacter baumannii的TolQ-TolR内膜蛋白复合物的结构结构

Elina Karimullina1,2, Yirui Guo3,4, Hanif M Khan5

  • 1Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada.

Science advances
|April 4, 2025
PubMed
概括

格拉姆阴性细菌使用质子动力 (PMF) 来保持细胞外完整性. 这是一个TolQ-TolR复合体.

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Structure of Porins01:21

Structure of Porins

Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a  motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
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Sec61 protein conducting channel
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In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
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