利用微塑料和塑料圈来监测废水废水排放到地表水中的人类致病细菌
Luke Woodford1, Lauren F Messer1, Michael J Ormsby1
1Biological and Environmental Sciences, Faculty of Natural Sciences, University of Stirling, Stirling, FK9 4LA, UK.
Water research
|April 4, 2025
概括
这项研究引入了一种新的微粒生物膜系统,用于在废水中检测可行的人类病原体. 该系统有效监测地表水中的病原体存在和毒性,帮助评估环境健康风险.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生监督是对公共卫生的监督.
- 废水工程 废水工程
背景情况:
- 污水处理厂 (WWTP) 的排放将人类病原体和抗菌素耐药性基因引入水生环境.
- 目前采集样本的方法不足以评估表面水中的病原体的生存能力和风险.
研究的目的:
- 开发和验证一种新的,低成本的基于微粒的生物膜系统,以加强对WWTP废水中可活性的人类病原体的监测.
- 评估WWTP下游的病原体的持续存在,度和毒性.
主要方法:
- 在WWTP废水管道上游和下游部署低成本微粒 (聚乙烯,软木,).
- 利用基于定量培养的分子方法 (16S rRNA基因测序) 和长读全基因组测序.
- 使用Galleria mellonella感染模型评估病原性.
主要成果:
- 可活性的大肠杆菌,克莱布西拉种类,花菌种类和菌种类. 在24小时内被检测到,并在整个23天的部署中持续存在.
- 病原体度在WWTP废水下游较高.
- 生物膜含有毒性和抗菌素耐药性基因;病原体在"塑圈"中仍然具有高度毒性.
结论:
- 微粒子生物膜系统提供了一种有效的,易于部署的方法,用于快速检测点源污染的可活性病原细菌.
- 这种监测方法可以改善环境管理,并减少与WWTP排放相关的公共卫生风险.
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