关于在非繁殖季节持续高温对大鱼的潜在影响的研究
Yue Chen1, Jun Wang2, Chun Xiu Jin1
1School of Marine Biology and Fisheries, Sanya Nanfan Research Institute of Hainan University, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, China.
概括
高水温对鱼繁殖产生负面影响,导致卵巢细胞亡并改变基因表达. 这表明在水产养殖中丸发育和后代生产存在潜在风险.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 鱼类生物学 鱼类生物学
- 环境压力生理学环境压力生理学
背景情况:
- 水产养殖的扩张需要了解鱼类对环境变化的反应.
- 全球气温上升对水生生态系统和水产养殖生产构成重大威胁.
研究的目的:
- 为了研究模拟高水温对大鱼生理学和分子机制的影响.
- 评估非繁殖季节对生长,繁殖,新陈代谢,亡和甲基化的影响.
主要方法:
- 在28天内将大鱼暴露在28°C的温度下.
- 分析生长,激素水平,基因表达 (转录组) 和甲基化标志物.
- 检查氧化应激和内质网膜应激指标.
主要成果:
- 高温降低了生长激素和雌激素,提高了女性的皮质醇,改变了生殖基因的表达.
- 转录组分析揭示了显著的基因表达变化和ECM受体相互作用和PPAR信号通路的丰富.
- 在女性中观察到氧化应激增加,内分泌网膜应激和卵巢细胞亡.
- 男性的甲基化模式发生变化 (高5甲基和甲基转移酶,低脱甲基酶),可能会影响丸发育.
结论:
- 持续的高温会通过荷尔蒙变化,基因表达改变和内质网膜压力诱导的亡来破坏大嘴的卵巢发育.
- 男性的表观遗传修饰表明,高温可能会损害丸的发育和随后的后代生产.
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