来自Botrytis cinerea的细胞死亡诱导蛋白BcPlp1有助于致病性并调节植物耐药性
Xiaofei Nie1, Ziyao Wang1, Binbin Huang1
1School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
概括
Botrytis cinerea分泌一种诱导细胞死亡的蛋白质,BcPlp1,它触发了植物细胞死亡并增强了耐药性. 这种蛋白质的蛋白质.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 在真菌病原体的产生过程中.
背景情况:
- Botrytis cinerea通过灰色的菌和腐烂造成了大量的作物损失.
- 病原体分泌的细胞死亡诱导蛋白 (CDIP) 促进植物组织死亡.
- 在Botrytis cinerea-植物相互作用中,CDIP诱导的细胞死亡的机制尚未完全理解.
研究的目的:
- 为了研究Botrytis cinerea CDIP BcPlp1在植物细胞死亡诱导中的作用和机制.
- 确定参与BCPlp1活动的关键结构元素和宿主因素.
- 为了阐明BcPlp1对Botrytis cinerea病原性的贡献.
主要方法:
- 净化和应用GST标记的BcPlp1融合蛋白.
- 评估各种植物物种的细胞死亡诱导.
- 分析特定的氨酸残留物和α螺旋结构的作用.
- 研究氨酸丰富的重复 (LRR) 受体类激酶 (BAK1和SOBIR1) 的参与.
- 评估BcPlp1删除和过度表达对真菌特征和毒性的影响.
主要成果:
- BcPlp1被分泌到植物的细胞囊中,并诱导细胞死亡.
- 四个半氨酸残留物和一个N端的α螺旋对BcPlp1的细胞死亡活动至关重要.
- 纯化BcPlp1触发了植物细胞死亡,调高了防御基因,并增强了对B. cinerea的抵抗力.
- BcPlp1的活性是由LRR受体类激酶BAK1和SOBIR1.1调节的.
- BcPlp1对真菌生长或承受压力无关紧要,但在过度表达时有助于致病性.
结论:
- BcPlp1是Botrytis cinerea的一个关键分泌的效应蛋白,直接诱导植物细胞死亡.
- BcPlp1诱导细胞死亡的活动取决于特定的结构特征和宿主受体激酶.
- BcPlp1在Botrytis cinerea的致病性中起着重要作用,特别是当其表达增加时.
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