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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Photochemical Electrocyclic Reactions: Stereochemistry01:26

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The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
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Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
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Production and Characterization of Vacuum Deposited Organic Light Emitting Diodes
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在延长的温度下,明亮的上升转换是有机表面层所能实现的.

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我们在使用有机分子的兰化物合纳米晶体中增强了光子上转换发光. 这一策略提高了高温的效率,并使新的热色应用成为可能.

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科学领域:

  • 材料科学 材料科学 材料科学
  • 纳米技术纳米技术
  • 光子学是指光子学的使用方法.

背景情况:

  • 兰化物合的纳米晶体提供可调节的光子频率向上转换.
  • 低发光效率和热火限制了它们的实际用途.
  • 表面缺陷和高能振荡器有助于能量消耗.

研究的目的:

  • 为了提高在广泛的温度范围内的上转换发光效率.
  • 通过表面修饰来研究发光增强的机制.
  • 开发适用于先进应用的适应温度的升级转换材料.

主要方法:

  • 在NaGdF4:Yb3+/Tm3+纳米晶体上,小型有机分子的表面协调.
  • 在不同温度和湿度下对上转换发光特性进行表征.
  • 涉及缺陷被动化和表面振荡器隔离的机制研究.

主要成果:

  • 有机表面协调显著提升了光的转换.
  • 增强效应随着温度的增加而加剧,特别是在潮湿的条件下.
  • 观察到对热刺激的异常光学反应,使热色切换成为可能.

结论:

  • 表面协调是一种多功能策略,可以克服热火并提高上转换效率.
  • 开发的材料具有适用于防伪和逻辑加密的温度依赖光学特性.
  • 这项工作为设计具有定制光学反应的先进功能纳米材料开辟了道路.