葡萄糖溶解模型表明,菌保持高ATP,并限制中间体的积累
Mangyu Choe1, Tal Einav2, Rob Phillips3
1Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, California; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California.
Biophysical journal
|April 5, 2025
概括
这项研究揭示了糖解如何控制细胞能量 (ATP) 水平. 质量作用调节ATP的产生,而全osteric反可以防止有害的代谢中间体的积累.
科学领域:
- 生物化学 生化学
- 系统生物学 系统生物学
- 代谢调节 代谢调节 代谢调节 代谢调节
背景情况:
- 葡萄糖分解是ATP和前体生产的基本代谢途径.
- 糖解酶维持ATP平衡的精确机制尚未完全理解.
- 主要的平衡作用任务包括调节产量的速度,保持稳定的ATP水平,控制中间度.
研究的目的:
- 为了研究质反和质量作用在哺乳动物糖解中的作用.
- 开发人类糖解的生物物理模型,以复制ATP平衡.
- 为了预测糖溶性中间体度和同位素追踪动力学.
主要方法:
- 人类糖解的生物物理模型的开发.
- 使用从体外动力学数据中得出的酶速率方程.
- 将模型预测与人类细胞中的实验测量进行比较.
主要成果:
- 该模型成功地回顾了ATP稳态,并预测了细胞代谢物度和同位素追踪动力学.
- 仅仅质量作用就足以控制ATP的产生速度和ATP水解的能量.
- 对于维持高ATP水平和防止中间积累而言,基酶和酸果基酶的整体调节至关重要.
结论:
- 体反确保酶活性与ATP需求相匹配,防止过度的葡萄糖酸化.
- 这种调节通过平衡ATP生产和消费来维持新陈代谢平衡.
- 开发的方法为了解代谢控制提供了定量框架.
相关概念视频
Glycolysis: Preparatory Phase
13.1K
In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
13.1K
ATP Energy Storage and Release
9.0K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
One example of energy coupling using ATP involves a...
9.0K
Glycolysis: Pay-off Phase
9.7K
So far, glycolysis has cost the cell two ATP molecules and produced two small, three-carbon sugar molecules. These molecules will proceed through the second half of the pathway, and sufficient energy will be extracted to pay back the two ATP molecules used as an initial investment and produce a profit for the cell of two additional ATP molecules and two even higher-energy NADH molecules.
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
9.7K
Outcomes of Glycolysis
98.5K
Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
98.5K
What is Glycolysis?
163.3K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
163.3K
Fates of Pyruvate
8.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.3K


