相关实验视频

Updated: May 16, 2025

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

量化纸张喷雾质谱学的内部标准利用策略

Lucas R Abruzzi^1,2, John-Clare Laxton^1,2, Taelor M Zarkovic^1,2

¹Department of Chemistry, Vancouver Island University, Nanaimo, BC V9R 5S5, Canada.

Journal of the American Society for Mass Spectrometry

|April 5, 2025

概括

优化内部标准 (ISTD) 对于纸张喷雾质谱 (PS-MS) 是至关重要的. 预存ISTD,特别是自动化,显著提高了定量分析的精度和准确性,满足了法医科学标准.

科学领域:

分析化学分析化学
质谱测量质量谱测量
法医科学法医科学法医科学

背景情况:

预混合内部标准 (ISTD) 与纸张喷雾质谱仪 (PS-MS) 的样本是低效的,不适用于全世界.
虽然独立的ISTD在纸上存储是可行的,但可能会损害定量准确性和精确性.

研究的目的:

评估PS-MS的不同ISTD利用策略,以确定不可重复性和偏差的来源.
为了比较预混合,预存和后存的ISTD方法的定量性能.
评估机器人与手动管道对ISTD沉积精度的影响.

主要方法:

研究的ISTD沉积策略 (预混合,预存,后存) 使用滥用药物作为分析物.
基于精度 (CV) 和准确度 (bias) 的评估性能,具有不同的ISTD卷.
比较机器人液体处理系统与手动管道用于ISTD沉积.
利用策略匹配的校准来纠正系统偏差.

主要成果:

预混合的ISTD产生了最好的结果 (平均CV = 1%, 置 = 5%),显著优于独立存入的ISTD (平均CV = 18%, 置 = 61%).
使用机器人处理器预存的ISTD在较低的体积下显示出最佳性能 (CV = 8%为2μL).
手动输入低量预先存入的ISTD导致性能差,而后期存入的ISTD通常较差.

结论:

ISTD的预置,特别是使用自动化系统,提高了PS-MS的定量性能.
策略匹配的校准有效地纠正了不同ISTD利用方法中的偏差.
在大多数评估场景中达到美国法医科学院 (AAFS) 的准确性和精度要求.

关键词:

我们的内部标准是内部标准.处理液体样本处理液体样本处理纸张喷雾质谱仪质谱仪.医疗服务点的分析.样品的准备样品的准备

更多相关视频

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

相关概念视频

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...