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扩大生物膜生物反应器的规模,以提高menaquinone-7的产量

Aydin Berenjian^1,2, Ehsan Mahdinia³, Ali Demirci⁴

¹Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, 80523, USA.

Bioprocess and biosystems engineering

|April 5, 2025

概括

使用生物膜反应堆扩大menaquinone-7 (MK-7) 的生产是可行的. 这项研究成功地将该过程扩大到30-L,实现了高MK-7产量,特别是在养批量策略下.

科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
营养科学营养科学

背景情况:

menaquinone-7 (MK-7) 具有显著的健康益处,通常通过发酵产生.
现有的MK-7生产方法面临着可扩展性挑战.
使用Bacillus subtilis natto的生物膜反应器为MK-7生物合成提供了一种新的方法.

研究的目的:

扩大一个创新的生物膜反应器,用于MK-7生产,从2L扩大到30L.
为了评估氧气质量转移,输入功率和推进器尖端速度对MK-7产量的影响.
调查料批量策略在试点规模反应堆中增强MK-7生产的有效性.

主要方法:

在塑料复合材料支上使用Bacillus subtilis natto开发并扩展了一个生物膜反应器.
评估了三个扩展策略:恒定体积氧气质量转移系数 (k_La),每单位体积的振动输入功率 (P/V) 和推进器尖端速度 (V_tip).
在30L试验级反应堆中实施了料批次葡萄糖添加策略.

主要成果:

在扩展过程中保持恒定的k_La被证明是MK-7生产中最有效的,在30-L时达到21.0 ± 1.0 mg/L.
食批量策略显著提高了MK-7度,达到28.7 ± 0.3 mg/L,比悬浮细胞生物反应器增加了2.3倍.
生物膜反应器技术成功展示了可扩展性和商业MK-7生产的潜力.

结论:

生物膜反应器技术可用于商业MK-7生产.
保持一致的k_La对于成功的扩展至关重要.
在生物膜反应堆中,储存批量策略在增加MK-7产量方面具有显著的优势,超过了传统方法.

关键词:

生物膜反应器的反应器商业生产的商业生产.梅纳金-7是什么意思扩大规模 - 扩大规模量度质量转移系数的体积转移系数

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