隔离,选和优化Endophytic细菌的抗氧化剂生产从Zingiber officinale罗斯科 (生)
Trang Thi Xuan Dai1, Tuan Thanh Chau2, Linh Chi Tran3,4
1Department of Biology, College of Natural Sciences, Can Tho University, Campus II, 3-2 Street, Ninh Kieu District, Can Tho City, 94000, Viet Nam. dtxtrang@ctu.edu.vn.
Current microbiology
|April 5, 2025
概括
生中的内菌,特别是Kosakonia sp. 的内菌. ZO-Rh4,显示出高的抗氧化能力. 优化的发酵条件显著提高了抗氧化剂化合物生产,突出了它们作为天然抗氧化剂的用途.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 自然产品 化学 化学
背景情况:
- 生 (Zingiber officinale Roscoe) 是一种传统的草药,具有药用.
- 在药用植物中存在的内生细菌可以产生生物活性化合物.
- 抗氧化剂对于对抗氧化应激和相关疾病至关重要.
研究的目的:
- 从具有高抗氧化能力的生中分离和识别内菌.
- 为了优化发酵条件,通过选定的菌株增强抗氧化剂的生产.
- 为了评估细菌代谢物的抗氧化能力.
主要方法:
- 从生组织中分离和选内菌的细菌.
- 使用16S rRNA基因测序识别有前途的菌株.
- 优化发酵参数 (温度,时间,葡萄糖,pH).
- 总含量 (TPC) 和总黄含量 (TFC) 的量化.
- 对抗氧化活性进行检测 (DPPH清除,总抗氧化能力,降低功率).
主要成果:
- 32种内菌株表现出显著的抗氧化活性.
- 科萨科尼亚 sp. 科萨科尼亚 sp. 在已识别的菌株中,ZO-Rh4显示出最高的抗氧化潜力.
- 优化的发酵条件 (32.58°C,71.49h,14.45 g/L葡萄糖,pH 7.58) 导致TPC (285.74 mg GAE/mL) 和TFC (144.49 mg QE/mL) 的高水平.
- 通过各种测试证实了增强的抗氧化活性.
结论:
- 内生菌的细菌,特别是Kosakonia sp. 这种细菌. 中的ZO-Rh4是一种有前途的天然抗氧化剂来源.
- 优化发酵显著提高了抗氧化化合物的产生.
- 这些发现支持生衍生的内细胞作为天然抗氧化剂的潜在应用.
相关概念视频
00:57Antibiotic Selection
Overview
01:20Endospores and Sporulation
Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...
01:25Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
01:27Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
01:27Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...