死亡细胞的歧视与红色发射碳量子点从药用和食用草本Echinophora tenuifolia
Naciye Ozdemir1, Gamze Tan2, Atakan Tevlek3
1Department of Biochemistry, Faculty of Science, Selçuk University, Konya, 42075, Turkey.
Journal of fluorescence
|April 5, 2025
概括
绿色合成的红色发射碳量子点 (CQD) 来自Echinophora tenuifolia选择性地染色生物成像的死细胞. 这些生物相容的CQD提供了卓越的光稳定性和抗氧化能力,在细胞活力研究中具有潜在的应用.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 绿色化学 绿色化学
背景情况:
- 准确的细胞活力测定对于体外研究至关重要.
- 基于光的活/死细胞染色是评估细胞健康的首选方法.
- 碳量子点 (CQD) 正在成为生物成像的有希望的工具.
研究的目的:
- 开发一种绿色合成方法,用于来自Echinophora tenuifolia的红色发射CQD.
- 评估这些CQD的生物相容性,光稳定性和抗氧化能力.
- 研究CQD在选择性活/死细胞染色和生物成像方面的潜力.
主要方法:
- 红色发射CQDs的绿色合成使用微波辐射Echinophora tenuifolia.
- 通过DPPH,ABTS,CUPRAC,FRAP,PBD和MCA测定对CQD抗氧化能力的评估.
- 对L929和HepG2细胞系的细胞毒性评估和泽塔电位测量.
- 不同染色实验和光显微镜用于活/死细胞成像.
主要成果:
- 从E. tenuifolia合成的CQD显示出优越的生物相容性和光稳定性.
- CQD证明了与植物提取物相比的抗氧化能力.
- CQD显示对HepG2细胞具有选择性毒性,但对L929细胞没有.
- 一个强大的负泽塔潜力通过对活细胞的排斥来促进死细胞的选择性染色.
- 确定非毒性成像的最佳度为25μg/mL,在德克萨斯州红色过器下观察到红色光.
结论:
- 来自E. tenuifolia的红色发射CQDs使用绿色微波辅助方法有效合成.
- 这些CQD具有有利于生物成像的特性,包括高光稳定性和选择性死细胞染色.
- 在细胞活力研究中,CQD显示了可靠的长期生物成像应用的潜力.
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