相关实验视频

Updated: May 16, 2025

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

脑性树冠病毒转移受体识别的分子基础

Xiaoyi Fan¹, Wanyu Li¹, Jessica Oros¹

¹Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.

|April 5, 2025

概括

西方马脑炎病毒 (WEEV) 已经转移了受体结合. 了解这些变化是预测和准备未来WEEV疫情的关键.

科学领域:

病毒学
结构生物学
免疫学

背景情况:

西方马脑炎病毒 (WEEV) 历来在美洲引起了严重的脑炎疫情.
作为受体,WEEV使用原素10 (PCDH10) 和低密度脂蛋白受体 (LDLR) 相关的蛋白质.
最近的WEEV分离体对哺乳动物受体的结合减少,有利于鸟类受体.

研究的目的:

阐明WEEV变化的受体热带的结构基础.
通过理解受体切换来评估WEEV再次出现的风险.
识别潜在的治疗点对抗WEEV.

主要方法:

确定了WEEV与人类PCDH10,禽类PCDH10和人类极低密度脂蛋白受体 (VLDLR) 的结合结构.
分析了E2糖蛋白替代对哺乳动物受体结合的影响.
在小鼠模型中评估可溶性VLDLR片段的疗效.

主要成果:

一到三次E2糖蛋白替代使非致病性WEEV菌株能够与哺乳动物的受体结合.
结构分析显示了WEEV与受体相互作用的关键变化.
在小鼠中,可溶性VLDLR片段提供了对致命WEEV的保护.

结论:

特定的E2糖蛋白突变可以恢复WEEV中的哺乳动物受体结合.
在机理上理解WEEV的受体热带变化并且可能是可逆的.
这些发现对于WEEV疫情的准备工作至关重要,特别是考虑到最近南美再次出现的疫情.

关键词:

阿尔法病毒植物病毒新出现的病毒大脑炎接收器病毒糖蛋白

更多相关视频

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

Published on: May 29, 2016

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

相关概念视频

Cross-reactivity

Cross-reactivity

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...