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从乳杆菌中分离和表征抗微生物:探索作用机制

Mahsa Niknam¹, Leila Sadeghi¹, Gholamreza Zarrini¹

¹Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

Microbial pathogenesis

|April 5, 2025

概括

来自Lactobacillus sp.的新型抗微生物 (AMP) 是一种新型抗微生物. 通过破坏膜和抑制DNA聚合酶,对抗性细菌表现出强烈的活性. 这些,BAP I和BAP III,为新的感染治疗提供了双重作用的潜力.

科学领域:

微生物学微生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

抗生素耐药性是一个日益增长的全球健康威胁,需要新的治疗策略.
抗微生物 (AMP) 是一类具有广谱活性的有前途的化合物.
乳酸菌种是已知的生物活性分子的生产者,包括潜在的AMP.

研究的目的:

从Lactobacillus sp.中分离和描述新的抗微生物.
评估净化的抗菌活性和作用机制.
为了研究这些的潜力作为对抗多药耐药细菌的药物.

主要方法:

使用凝过色谱 (GFC) 隔离和净化.
通过MALDI-TOF MS和SDS-PAGE进行表征.
评估抗菌活性对抗格拉姆阳性和格拉姆阴性细菌.
使用FE-SEM成像对膜破坏的分析.
通过PCR对DNA聚合酶抑制的评估.
使用循环二元化 (CD) 光谱学的合规变化分析.
分子对接和动力学模拟.

主要成果:

两个生物活性,生物活性I (BAP I, 4168.14 Da) 和生物活性III (BAP III, 8076.45 Da),被分离和净化.
这两种都对Pseudomonas aeruginosa,Streptococcus sanguinis,Bacillus cereus和Staphylococcus aureus表现出强大的抗菌活性,其中BAP I的疗效更高.
FE-SEM成像证实了质破坏细菌膜.
BAP I和BAP III抑制了细菌DNA聚合酶的活性,PCR产物形成减少的证据.
CD光谱检测显示,结合诱导了Taq聚合酶的构造变化,增加了它的灵活性.
分子对接和动力学研究表明与DNA聚合酶的稳定相互作用,表明作用的双重机制.

结论:

BAP I和BAP III通过双重机制显示出显著的抗菌潜力:细菌膜破坏和DNA聚合酶的抑制.
BAP I的两结构和水性C端区域有助于其增强的疗效.
这些代表了开发针对多药耐药性感染的新疗法的有希望的候选人.
需要进一步的研究来评估现有抗生素的安全性,临床疗效和协同潜力.

关键词:

抗微生物是一种抗微生物.抑制DNA聚合酶的抑制作用凝过色谱学凝过色谱学牛乳杆菌 (Lactobacillus) 是一种细菌.马尔迪-托夫 (MALDI-TOF) 是一个国家元首.酸净化净化方法

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