弗拉盖林工程通过重塑瘤微环境在固体瘤中来增强CAR-T细胞功能.
Xiangyun Niu1,2, Pengchao Zhang1,2, Liujiang Dai1
1Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, Shenzhen, Guangdong, China.
Journal for immunotherapy of cancer
|April 5, 2025
概括
工程化化学抗原受体 (CAR) - - 表达沙门氏菌鞭毛素 (Fla) 的T细胞可以重塑瘤微环境 (TME). 这一策略通过激活免疫细胞和促进内源性T细胞反应来提高CAR-T细胞对固体瘤的疗效.
科学领域:
- 免疫学 免疫学 免疫学
- 癌症治疗 癌症治疗
- 细胞工程 细胞工程
背景情况:
- 使用仿真抗原受体 (CAR) - - T 细胞的采用细胞疗法在血液恶性瘤中显示出有前途.
- 免疫抑制性瘤微环境 (TME) 限制了CAR-T细胞在固体瘤中的疗效.
- 重塑TME的策略对于加强固体瘤中CAR-T细胞治疗至关重要.
研究的目的:
- 设计CAR-T细胞以表达沙门氏菌鞭毛素 (Fla) 来激活免疫细胞并重塑TME.
- 评估Fla工程CAR-T细胞在固体瘤中的治疗潜力.
主要方法:
- 卡尔-T细胞被设计成表达沙门氏菌旗素 (Fla).
- 在共同培养和小鼠瘤模型中进行了功能验证.
主要成果:
- Fla-engineered CAR-T细胞激活了与瘤相关的巨细胞和树突细胞,将TME转化为一个"免疫热"的环境.
- 这种免疫调节增强了CAR-T细胞功能,并改善了对目标阳性固体瘤的控制.
- Fla表达还促进了内源性细胞毒性CD8+T细胞的产生,增强了对抗原异质性瘤的疗效.
结论:
- 沙门氏菌旗素 (Fla) 可以通过重塑TME来重新连接抗瘤免疫力.
- 设计CAR-T细胞来表达Fla是一种可行的策略,可以提高对固体瘤的治疗疗效.
相关概念视频
01:27Tumor Immunotherapy
441
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
441
02:17The Tumor Microenvironment
6.5K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.5K