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RNA-Seq分析揭示了与野生Myotis myotis蝙蝠免疫相关的长非编码RNA

Sebastien Riquier¹, Samuel Carthy¹, Graham M Hughes¹

¹School of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.

|April 5, 2025

概括

由于独特的免疫系统,蝙蝠在没有疾病的情况下接收病毒. 研究人员在蝙蝠中确定了长非编码RNA (lncRNAs),这些RNA可以调节对病毒和炎症的免疫反应.

科学领域:

病毒学病毒学
基因组学就是基因组学.
免疫学免疫学免疫学

背景情况:

蝙蝠对病毒感染具有显著的弹性,这种特征与它们独特的免疫系统有关.
控制蝙蝠免疫反应的精确分子机制,特别是关于病毒病原体,仍然不完全理解.
长非编码RNAs (lncRNAs) 越来越多地被认为是它们在生物过程中的关键调节作用,包括免疫力.

研究的目的:

研究 lncRNAs 在蝙蝠免疫系统调节中的作用.
为了确定参与管理蝙蝠病毒感染和炎症的特定lncRNAs.
假设 lncRNA 调节有助于蝙蝠与病毒病原体共存的能力.

主要方法:

在多个蝙蝠组织中进行转录组注释的 lncRNA 预测管道的开发.
分析了来自野生Myotis myotis蝙蝠的100个血液转录组.
权重基因共同表达网络分析 (WGCNA) 和基因本体学的应用,用于识别mRNA-lncRNA共同表达模块.

主要成果:

蝙蝠 lncRNAs的表征揭示了较低的,组织特异性的表达,减少的GC含量和较短的长度与编码基因相比.
在Myotis myotis中发现了两个与T细胞激活/病毒过程和炎症相关的mRNA-lncRNA联合表达模块.
包括新型BatLnc1,M. myotis TUG1,MALAT1和NEAT1在内的四个候选免疫相关的lncRNA因其在抗病毒和抗炎功能中的潜在作用而被选择.

结论:

这项研究提供了第一个对非模型蝙蝠物种Myotis myotis的lncRNAs的*ab initio*预测.
网络分析表明,个体之间的免疫状况存在差异,可能与疾病有关.
鉴定到的lncRNA与蝙蝠的免疫反应密切相关,为未来的功能研究和对蝙蝠抗病毒策略的洞察提供了基础.

关键词:

生物信息学是一种生物信息学.血液中的RNA是什么?长腿动物 (Chiroptera) 是一个类动物.豁免权是一种豁免权.炎症炎症是一种炎症.文字转录学 (Transcriptomics) 是一个学科.病毒病毒病毒

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