在Saccharomyces cerevisiae Rtt103和Rat1-Rai1复合体之间的相互作用的分子基础
1Department of Biological Sciences, Columbia University, New York, NY, USA.
Nature communications
|April 5, 2025
概括
在Rat1-Rai1-Rtt103复杂结构中,可以发现Rtt1033.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生化学
背景情况:
- 鼠1 5'-3'外核糖酶和Rai1对于Saccharomyces cerevisiaeRNA聚合酶II的转录终止至关重要.
- Rtt103蛋白与Rat1-Rai1共净化,但其相互作用机制尚不清楚.
研究的目的:
- 为了确定Saccharomyces cerevisiae Rat1-Rai1-Rtt103三元复合物的冷电子显微镜 (cryo-EM) 结构.
- 阐明Rtt103与Rat1-Rai1.1.3相互作用的分子机制.
主要方法:
- 低温电子显微镜 (cryo-EM) 在2.9 Å分辨率.
- 位点定向的突变发生 (Rtt103中的P261G/Y263A突变).
主要成果:
- 冷EM结构显示了Rat1-Rai1-Rtt103.3的三元复合体.
- Rtt103的一个特定细分,称为Rai1交互细分 (RIS),直接与Rai1.1联系.
- 在RIS (P261G/Y263A) 中的突变破坏了与Rat1-Rai1.1结合的Rtt103.
结论:
- Rtt103 的 Rai1 相互作用部分对于其与 Rat1-Rai1 综合体的关联至关重要.
- 这种相互作用可能会促进Rat1-Rai1对RNA聚合酶II的招募,以在酵母中终止转录.
- 这些发现与之前在其他酵母菌种中观察到的相互作用形成鲜明对比,突出了特定物种的机制.
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