相关实验视频

Updated: May 17, 2025

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Published on: March 2, 2017

单细胞转录学揭示了人类诱导的肝细胞样细胞的血统重编程期间的肝脏发育轨迹

Nan Jiang^1,2, Guangya Li³, Sen Luo^1,2

¹Laboratory of Neurological Diseases and Brain Function, the Affiliated Hospital, Southwest Medical University, Luzhou, China.

Cellular and molecular life sciences : CMLS

|April 6, 2025

概括

这项研究揭示了关键的基因调控网络 (GRNs),用于产生功能性人类诱导肝细胞 (hiHeps). 了解这些网络,包括转录因子HNF4A和HHEX,可以改善治疗用途的hiHeps生产.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
遗传学遗传学是一个

背景情况:

在体外产生功能性肝细胞对于药物查,疾病建模和移植至关重要.
建立真正的肝细胞基因调节网络 (GRNs) 是体外的一个重大挑战.
一个两步的谱系重编程策略已经开发出来,以产生人类诱导的肝细胞 (hiHeps).

研究的目的:

探索使用单细胞RNA测序 (scRNA-seq) 在hiHeps生成期间的细胞命基转换.
确定参与建立肝细胞特异性特征的关键基因调节网络 (GRNs).
了解功能性部的高效生产的基础机制.

主要方法:

高通量单细胞RNA测序 (scRNA-seq) 用于在重新编程过程中分析细胞群.
在单细胞分辨率下研究了转录动态和细胞酸盐轨迹.
确定和分析了表面标记物 (CD24,DLK1) 和转录因子 (HNF4A,HHEX).

主要成果:

重编程的后期阶段与类似的转录波反映了自然肝脏发育.
CD24和DLK1标志着不同的肝脏原始体种群.
脂质代谢增强hiHeps成熟,HNF4A/HHEX控制细胞命运的决定.

结论:

这项研究提供了在hiHeps诱导过程中对肝细胞特异性GRN建立的单细胞分辨率洞察.
这些发现有助于更有效地生成功能性肝细胞,用于治疗应用.
了解细胞命运决策和成熟途径是再生医学的关键.

关键词:

基因监管网络是基因监管网络.人类诱导的肝细胞.血统重编程是一种重编程.脂质新陈代谢脂质新陈代谢单细胞RNA测序的一个细胞.

更多相关视频

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Forced Transdifferentiation

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...