使用纳米流液体染色学-协同质谱学双重代谢学和蛋白质学协议
Weiwei Lin1, Fatemeh Mousavi2, Benjamin C Blum2
1Center for Network Systems Biology, Boston University, Boston, MA 02118, USA; Department of Pharmacology, Physiology & Biophysics, Boston University Chobanian & Avedisian School of Medicine, Boston, MA 02118, USA.
STAR protocols
|April 6, 2025
概括
这项研究介绍了一种使用纳米流液体色谱-并联质谱 (nLC-MS) 进行双重代谢和蛋白质组学的新方案. 该方法提高了各种生物标本的灵敏度和样本清理.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 蛋白质组学和代谢组学
背景情况:
- 纳米流液体染色学-并联质谱学 (nLC-MS) 为非目标代谢学提供了增强的灵敏度.
- 从单个样本中整合蛋白质组学和代谢组学可以改善生物学见解.
- 毛细管柱阻塞是nLC-MS协议中常见的挑战.
研究的目的:
- 为基于nLC-MS的代谢学和蛋白质学同时提出一个精细的协议.
- 详细说明有效的代谢物清洁和丰富的方法.
- 为了在各种生物样本类型中实现强大的双体奥米克分析.
主要方法:
- 使用nLC-MS开发双重代谢学和蛋白质学协议.
- 实施固态微提取 (SPME) 用于代谢物清洁和丰富.
- 对nLC-MS数据采集和分析的详细程序.
主要成果:
- 在包括生物流体,细胞系和组织在内的各种标本中成功应用该协议.
- 在防止毛细血管柱堵塞方面,SPME的有效性已被证明.
- 能够对代谢物和蛋白质进行敏感和综合分析.
结论:
- 本协议有效地通过nLC-MS实现了双重代谢和蛋白质组.
- 在SPME辅助的清理对于协议的稳定性和可重复性至关重要.
- 这种方法为全面的生物样本分析提供了有价值的工具.
相关概念视频
Peptide Identification Using Tandem Mass Spectrometry
6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
Mass Spectrometry: Complex Analysis
660
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
660


