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Updated: May 11, 2026

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

对Listeria monocytogenes中毒性的比较分析:从基因组变异和体外基于细胞的研究中获得的见解

Yiyang Song¹, Binru Gao¹, Hua Cai²

¹School of Health Science and Engineering at the University of Shanghai for Science and Technology, China.

International journal of food microbiology

|April 6, 2025

概括

根据血统和来源,Listeria monocytogenes的毒性有所不同. 临床菌株和I系菌株显示出更强的侵入肠道和胎盘细胞的能力,这与特定的遗传因素有关.

科学领域:

通过食物传播的病原体
微生物的毒性微生物的毒性
基因组学就是基因组学.

背景情况:

李斯特菌 (Listeria monocytogenes) 是一种主要的食源性病原体,导致侵袭性李斯特菌病.
了解菌株特异性毒性对公共卫生至关重要.
以前的研究强调了细菌遗传学在病变发生过程中的重要性.

研究的目的:

来自上海的Listeria monocytogenes菌株之间的毒性差异的调查.
为了将体外毒性现象类型与全基因组数据相关联.
确定与增强的细菌入侵和转移相关的遗传因素.

主要方法:

从食品和临床样本中分离和表征37 L. monocytogenes菌株.
在体外评估肠道屏障转位 (Caco-2细胞).
在体外评估胎盘细胞 (JEG-3细胞) 的粘附和侵入.
全基因组测序和细菌菌株的分析.

主要成果:

与II系菌株相比,I系菌株的转移,粘附和入侵率明显高.
与IIa和IIc相比,血清组IIb菌株在JEG-3细胞中表现出更高的粘附性和入侵性.
临床分离物体的毒性明显大于来自食物的菌株.
在毒性较小的ST9和ST121菌株中发现了inlA基因的突变.
在ST87和ST3菌株中,LIPI-4和LIPI-3的存在与高毒性相关.

结论:

细菌的血统,血清组和起源 (食物与临床) 显著影响L. monocytogenes的毒性.
特定的遗传元素,如inlA突变和LIPI区域,有助于观察到的毒性差异.
将基因组数据与体外毒性试验相结合,可以全面了解病原体的行为.

关键词:

肠道屏障肠道屏障肠道屏障李斯特里亚单细胞原生菌 (Listeria monocytogenes) 是一种胎盘细胞是胎盘细胞.病毒性病毒性整个基因组测序的测序

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