在人类羊水样本中同时确定多种内分泌干扰化学物质,采用固相提取与液体染色体双重质谱法 (LC-MS/MS) 结合的方法
1School of Disaster and Emergency Medicine, Tianjin University, Tianjin, 300072, China; The First School of Medicine, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, China; Wenzhou Safety (Emergency) Institute, Tianjin University, Wenzhou, 325000, Zhejiang, China.
一种新方法准确地测量了羊水中的59种产前内分泌干扰化学物质 (EDC). 这种分析有助于了解胎儿暴露于环境污染物的风险,例如双和PFAS.
科学领域:
- 环境化学环境化学
- 毒理学 毒理学 毒理学
- 分析化学 分析化学
背景情况:
- 产前暴露于内分泌干扰化学物质 (EDC) 对后代健康构成风险.
- 胚胎液 (AF) 分析对于评估胎儿暴露于EDCs至关重要.
研究的目的:
- 开发和验证一种方法,用于同时确定59个EDC的人类AF中.
- 评估来自中国天津的AF样本中各种EDC的含量.
主要方法:
- 固态相提取 (SPE) 与液体色谱相结合 双重质谱 (LC-MS/MS).
- 同时确定11种双 (BPs),4种类甲 (parabens),11种类甲酸盐代谢物 (mPAEs),7种类有机酸盐代谢物 (mOPEs),8种类多环芳代谢物 (OH-PAHs) 和18种类高化合物 (PFASs).
- 方法验证包括对SPE参数,LOD,LOQ,回收和精度的调查.
主要成果:
- 该方法在一个小的AF体积 (500μL) 中成功确定了59个EDC.
- 达到检测 (LOD) 和量化 (LOQ) 的低极限 (0.02-0.2 ng/mL和0.07-0.66 ng/mL).
- 在中国天津的AF样本中检测到各种EDC的度,范围从
结论:
- 开发的LC-MS/MS方法灵敏,精确,适合分析人类AF中的多个EDC.
- 该方法证明了对现场样本的环保性和适用性.
- 这种分析工具有助于理解产前EDC暴露及其潜在的健康影响.
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