对MALDI-TOF MS和RAPD进行比较分析,以分组从亚临床乳腺炎分离出来的Streptococcus agalactiae
Ligia Beatriz Rizzanti Pereira1, Breno Luis Nery Garcia1, Carlos Eduardo Fidelis1
1Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Sciences, University of São Paulo, Pirassununga, 13653-900, SP, Brazil.
Microbial pathogenesis
|April 6, 2025
概括
矩阵辅助激光消化/离子化飞行时间质谱学 (MALDI-TOF MS) 有效地分组了乳腺炎中分离的Streptococcus agalactiae. 这种方法与抗菌素敏感性有很好的相关性,为乳牛群提供了快速诊断工具.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌学 细菌学是一门学科.
- 诊断技术 诊断技术的使用
背景情况:
- 银杆菌 (Streptococcus agalactiae) 是一种传染性病原体,在乳制品动物中引起亚临床乳腺炎.
- 准确的鉴定和表征对于管理S. agalactiae疫情至关重要.
- 抗微生物敏感性分析对乳腺炎的治疗决策有帮助.
研究的目的:
- 为了评估S. agalactiae分离物的分组,进行矩阵辅助激光吸/电离飞行时间质谱 (MALDI-TOF MS).
- 将抗微生物敏感性概况与MALDI-TOF MS分组联系起来.
- 为了比较MALDI-TOF MS分组与随机放大多态DNA (RAPD) 聚类.
主要方法:
- 在三个农场收集了198个牛和水牛的牛奶样本.
- 对10种使用最小抑制度 (MIC) 的抗微生物药物进行了抗微生物敏感性测试.
- 使用MALDI-TOF MS和RAPD分析分组的S. agalactiae分离物.
主要成果:
- MALDI-TOF MS确定了8个不同的群体,成功地将所有分离物分组在一起.
- RAPD确定了33个集群,仅分组了100个孤立物种.
- 两种方法都显示,同一群的分离物具有相似的敏感性概况.
- 观察到对塞夫提乌尔,青素,西林,阿莫西林,塞夫基诺姆, gentamicin 和 cefoxitin 的高敏感性 (> 90%).
结论:
- MALDI-TOF MS是快速分组S. agalactiae的一个有价值的工具.
- MALDI-TOF MS分组与抗菌素敏感性模式保持一致.
- MALDI-TOF MS为乳腺炎研究中的病原体分组提供了可靠的RAPD替代方案.
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