通过整合转录学和基因网络分析来识别斑马鱼胚胎中醇真菌杀菌剂毒性的分子特征
Fatma Marghany1, Steve U Ayobahan2, Gabriela Salinas3
1Department Ecotoxicogenomics, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Schmallenberg, Germany; Department Evolutionary Ecology and Environmental Toxicology, Faculty Biological Sciences, Goethe University Frankfurt, Frankfurt, Germany; Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt.
Environmental pollution (Barking, Essex : 1987)
|April 6, 2025
概括
醇杀菌剂破坏真菌醇生物合成,并可能伤害鱼类. 这项研究确定了斑马鱼胚胎中的基因表达模式,表明这些杀菌剂的内分泌干扰等有毒影响.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 生态毒理学 生态毒理学
背景情况:
- 青类杀菌剂抑制真菌醇生物合成.
- 亚醇在水生生物,特别是鱼类中显示出内分泌干扰作用.
- 了解鱼类中醇毒性的分子机制对于风险评估至关重要.
研究的目的:
- 分析暴露于醇杀菌剂的斑马鱼胚胎中的转录组反应.
- 识别基因表达指纹,表明有毒作用,包括内分泌干扰.
- 为了确定固醇生物合成抑制剂的不良影响的分子特征.
主要方法:
- 进行了经过修改的斑马鱼胚胎毒性测试 (经合组织236指南).
- 胚胎暴露于迪芬可纳,环氧可纳和铁布可纳.
- 对提取的RNA进行了转录组分析和过度代表性分析.
主要成果:
- 杀菌剂诱导了斑马鱼胚胎中的度依赖转录组反应.
- 不同表达的基因与固醇生物合成和内分泌干扰有关.
- 一个特定的基因组被确定为固醇生物合成抑制剂毒性的分子特征.
结论:
- 基因表达指纹可以表明斑马鱼中固醇生物合成抑制剂的不良影响.
- 已识别的生物标志物可能有助于对内分泌活性进行监管前查.
- 斑马鱼胚胎模型是评估杀菌剂毒性的宝贵工具.
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