暴露于PFOS会通过依赖于线粒体的铁代谢损害猪卵细胞成熟和胚胎发育
Xiaoqing Sun1, Ran Teng1, Ning Xu1
1Jilin Agricultural University, Xincheng Street 2888, Changchun, 130118, China.
Environmental pollution (Barking, Essex : 1987)
|April 6, 2025
概括
暴露在 perfluorooctane sulfonate (PFOS) 中会通过破坏线粒体和诱导细胞死亡途径铁亡来损害哺乳动物卵细胞的成熟. 这项研究揭示了PFOS通过细胞损伤机制的生殖风险.
科学领域:
- 环境毒理学环境毒理学
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
背景情况:
- perfluorooctane sulfonate (PFOS) 是一种持久性环境污染物,具有已知的健康风险.
- 以前的研究表明,PFOS可能会对哺乳动物的生殖系统造成伤害,但机制尚不清楚.
- 由于其广泛使用和生物积累性质,了解PFOS的毒性至关重要.
研究的目的:
- 研究PFOS影响哺乳动物卵细胞成熟和胚胎发育的机制.
- 利用猪卵细胞作为一个模型系统来探索PFOS诱导的细胞变化.
- 阐明铁,自和线粒体功能障碍在PFOS毒性的作用.
主要方法:
- 猪卵细胞在体外暴露于PFOS.
- 使用SMART-seq分析基因表达变化.
- 评估了线粒体功能,脂质过氧化 (MDA水平),铁 (Fe2+) 水平和关键基因表达 (PCBP1,PCBP2,P53).
主要成果:
- 暴露于PFOS导致线粒体损伤,影响结构和功能,并影响过氧体生物发生.
- 增加的Fe2+和MDA水平表明铁和脂质过氧化.
- 在暴露于PFOS的卵细胞中观察到PCBP1,PCBP2和P53的mRNA表达减少.
结论:
- 在实验室中暴露于PFOS会导致猪卵细胞中的线粒体损伤.
- 这种损伤会诱导脂质过氧化,导致铁亡.
- 通过这些细胞通路,PFOS对卵细胞成熟和潜在的胚胎发育产生不利影响.
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