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在活细胞中使用相关Brillouin-Raman微光谱学对核力学进行无接触性表征

S Kerdegari¹, A A Passeri², F Morena³

¹Department of Physics, University of Genova, Genova, Italy; Istituto Italiano di Tecnologia, Genova, Italy.

Acta biomaterialia

|April 6, 2025

概括

核力学对细胞功能至关重要. 相对布里卢恩-拉曼微光谱法 (BRMS) 非侵入性地测量活细胞中的核刚性,揭示提取的核比完整的更软.

科学领域:

细胞和分子生物学细胞和分子生物学
生物物理学的生物物理.
生物技术是生物技术.

背景情况:

核力学调节细胞生理学,染色质可访问性和转录.
原子力显微镜 (AFM) 是次微米力学的标准,但受到活细胞的接触和细胞骨干扰的限制.
对孤立核的机械测量是常见的,但制备的影响尚不清楚.

研究的目的:

用AFM和Brillouin-Raman微光谱学 (BRMS) 来比较完整的皮肤纤维细胞和提取的核中的核力学.
评估侵入性制剂对核机械性质的影响.
评估BRMS作为核机械生物学的一种非侵入性工具.

主要方法:

相关布里卢恩-拉曼微光谱 (BRMS) 用于非侵入性的3D成像和机械/化学性质映射.
原子力显微镜 (AFM) 用于亚微米机械表征.
对完整纤维细胞与孤立细胞核的机械特性进行比较分析.

主要成果:

与完整的核相比,提取的核具有显著降低的刚性.
BRMS有效地识别了活细胞内的核机械特征,从细胞骨和细胞质干扰中脱而出.
对孤立核的AFM测量并不完全代表原生核力学.

结论:

核力学最好在原生细胞环境中进行评估,以保持平衡.
与传统的AFM相比,BRMS提供了对核力学更深入的见解,特别是在活细胞中.
建议BRMS作为核机械生物学研究的参考工具.

关键词:

航空飞行管理 (AFM)布里卢恩显微镜的显微镜机械生物学机械生物学核力学核力学核力学拉曼光谱法拉曼光谱法拉曼光谱法

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