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Updated: May 17, 2025

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通过RiPP酶SyncM和PirF的设计和生产经过凝结的循环

Yanli Xu1, Fleur Ruijne1, Manel Garcia Diez1

  • 1Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, The Netherlands.

Biomacromolecules
|April 7, 2025
PubMed
概括

使用RiPP酶创建了新的基因编码的抗生素模仿物. 这些新型的脂化宏环为发现新的抗菌药物来对抗抗生素耐药性提供了有希望的途径.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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