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通过Sysmex-XN预稀释模式在毛细血管微样中自动计数红细胞和白细胞.

Claus Vinter Bødker Hviid1,2, Vibeke Staun Christensen1, Klaus Rosenkilde Jensen1

  • 1Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.

International journal of laboratory hematology
|April 7, 2025
PubMed
概括

在Sysmex XN系列预稀释 (PD) 模式提供可靠的白细胞分析从小血样. 然而,红细胞参数的准确性不足以用于临床使用.

关键词:
生物化学 生物化学方法比较方法比较新生儿血液学 新生儿血液学精确的精确度可以说是精确的.红细胞是血液中的红细胞.白细胞是白细胞的组成部分.

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科学领域:

  • 临床实验室科学 临床实验室科学
  • 血液学 血液学 血液学
  • 儿科医学 儿科医学

背景情况:

  • 新生儿患者需要最小的血液体积进行血液学分析,这对标准实验室方法构成了挑战.
  • 在Sysmex XN系列预稀释 (PD) 模式允许使用小血量 (20-50μL) 进行血液学测量.

研究的目的:

  • 使用50μL微样本验证Sysmex XN系列PD模式的分析性能和预分析稳定性.
  • 评估PD模式对于分析低体积样本中的关键血液学参数的适用性.

主要方法:

  • 从成年EDTA血液中准备了微样 (50μL) 用于分析.
  • 评估了白细胞计数和红细胞参数的不准确性和准确性.
  • 评估了毛细血管采样,储存稳定性和气动管运输的影响.
  • 在一小群儿科患者中得到验证的结果.

主要成果:

  • 白细胞计数显示可接受偏差 (≤5.4%) 和不准确 (<3.5%).
  • PD模式在毛细血管采样和7小时稳定性方面表现出良好的分析性能.
  • 红细胞参数通常超过了可接受的偏差和不准确度限制.
  • 儿科样本偏差是在预测的间隔内.

结论:

  • PD模式在分析上适用于白细胞计数,提供良好的分析前稳定性.
  • 由于性能限制,不建议使用PD模式进行红细胞参数分析.
  • PD模式为特定的血液学监测需求提供了一个潜在的低容量替代方案.