揭露致毒性类物种导致玉米耳烂:对富蒙尼辛生产潜力的洞察
Harinder Singh1, Harleen Kaur2, Mandeep Singh Hunjan1
1Department of Plant Pathology, Punjab Agricultural University, Ludhiana, Punjab, India.
Frontiers in plant science
|April 7, 2025
概括
玉米中的Fusarium耳是由北印度的Fusarium verticillioides和Fusarium proliferatum引起的. Fusarium verticillioides产生了更多的fumonisins,特别是在PMH 2玉米杂交中,与疾病严重程度的增加相关.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 食品安全 食品安全
背景情况:
- 虫耳 (Fusarium ear rot,FER) 在全球范围内对玉米产量和质量产生重大影响.
- 致病性Fusarium物种,特别是F. verticillioides和F. proliferatum,是FER的主要贡献者.
- 这些真菌产生有害的真菌毒素,如烟素,对人类和动物的健康构成风险.
研究的目的:
- 确定与北印度玉米耳腐烂相关的有毒Fusarium物种.
- 在实验室条件下评估这些分离物的富蒙尼辛生产潜力.
- 确定玉米杂交物对菌毒素积累的影响.
主要方法:
- 从受感染的玉米中收集和分离的Fusarium物种.
- 使用VERTF-1/2,FUM1和FUM13原料进行PCR,以检测产生富蒙尼辛的潜力.
- 测序分析确定了Fusarium物种.
- 在玉米杂交品 (PMH1和PMH2) 上人工注射后,使用LC-MS/MS对量化fumonisin B1和B2水平进行量化.
主要成果:
- Fusarium verticillioides是主要的物种,Fusarium proliferatum也被确定.
- F. verticillioides 隔离物 (Fus 15) 显示出比 F. proliferatum 隔离物 (Fus 48) 更高的烟素产量.
- 在PMH 2玉米杂交中观察到显著增加的fumonisin积累,与增加的FER严重程度相关.
结论:
- 这项研究突出了北印度玉米中Fusarium物种的流行率和毒性能力.
- 在这个地区,Fusarium verticillioides 是一种主要的烟素生产者.
- 玉米杂交易感性在真菌毒素积累中起作用,为有针对性的管理策略提供信息.
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