相关实验视频

Updated: May 15, 2025

Mitochondrial Respiration Quantification in Yeast Whole Cells

Mitochondrial Respiration Quantification in Yeast Whole Cells

Published on: November 8, 2024

开发了针对线粒体的奎尔丁,用于从氧化应激中拯救塞尔托利细胞

Satrialdi¹, Cellina Pratiwi¹, Ryan Novia Khaeranny¹

¹Department of Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Indonesia.

Research in pharmaceutical sciences

|April 7, 2025

概括

使用带有素 (DQ) 的切丁载脂酶的向抗氧化疗法对男性不孕症有很大的前景. 低密度的DQ有效地减少了线粒体中的氧化应激,验证了这种方法.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
生殖生物学生殖生物学

背景情况:

氧化应激,反应性氧物种 (ROS) 和抗氧化剂之间的不平衡,损害了精子生成,导致男性不孕.
线粒体是抗氧化剂治疗的关键目标,因为它们产生最多的ROS,对受精能量至关重要.
针对性地向线粒体输送抗氧化剂对于有效治疗与氧化压力相关的男性不孕症至关重要.

研究的目的:

开发针对线粒体的新型混合纳米平台,用于切丁的输送.
调查 (DQ) 度对纳米平台特性和有效性的影响.
评估这些纳米平台在缓解TM4细胞中氧化应激方面的潜力.

主要方法:

使用水化方法制备了含奎尔丁的脂质体.
在不同的度中加入了dequalinium (DQ),以创建混合纳米平台.
评估了粒子大小,封装效率,泽塔潜力,细胞内贩运和体外抗氧化活性.

主要成果:

不同的DQ度对粒子大小,封装效率和线粒体向影响最小.
降低DQ密度降低了泽塔潜力.
只有低密度的DQ (Q-LipoDQ LD) 才能有效地减少TM4细胞中的氧化应激;高密度的DQ (Q-LipoDQ HD) 没有.

结论:

低密度DQ修饰脂质体 (LipoDQ LD) 显示出作为针对线粒体的纳米平台的巨大潜力.
这项研究验证了线粒体作为抗氧化剂治疗的关键点,用于打击氧化应激.
这些发现支持针对男性不孕症治疗的向纳米药物的开发.

关键词:

抗氧化剂是一种抗氧化剂.针对线粒体的药物输送系统是针对线粒体的.塞尔托利细胞是Sertoli细胞.治疗疗法治疗疗法

更多相关视频

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Published on: September 16, 2020

相关概念视频

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...