相关实验视频
Updated: May 20, 2025

09:43
Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
12.0K
增强血红素进口以在Pichia pastoris中合成活性髓蛋白和血红蛋白
Yuan Qian1,2,3,4, Xuan Wang1,2,3,4, Yang Liu1,2,3,4
1Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu China.
3 Biotech
|April 7, 2025
概括
这项研究通过改善血红素吸收来增强Pichia pastoris中的血红蛋白生产. 过度表达血红素的进口者显著提高了肌红蛋白和血红蛋白的产量,为宝贵的血红蛋白合成提供了新的策略.
科学领域:
- 生物技术和合成生物学
- 蛋白质的工程和生产.
背景情况:
- 血红蛋白和血红蛋白等血红蛋白结合蛋白对食品加工和医学至关重要.
- 皮奇亚牧草是生产这些蛋白质的常见宿主,但由于细胞内血供应不足,经常遭受低产量.
- 当血红蛋白稀缺时,不活跃的血红蛋白会积累,从而限制异质蛋白合成的效率.
研究的目的:
- 通过提高血红素吸收效率来增强Pichia pastoris细胞内血红素供应.
- 识别和验证有效的异质血红素进口商,以促进血红蛋白的生产.
- 增加特定血红蛋白的标位,猪骨髓蛋白 (PMB) 和大豆血红蛋白 (SHB).
主要方法:
- 开发了一种查方法,使用一种缺乏血红素的Pichia pastoris菌株 (HEMEX) 通过敲除HEM1基因来创建.
- 选了各种微生物,以寻找潜在的血进口者,并补充了外源血.
- 选择并过度表达了两个有效的异质血红素进口者Caflc1和Shu1.
- 在进口者过度表达后,量化了PMB和SHB标位的增加.
主要成果:
- 在合成受损时,HEMEX菌株表现出显著改善的细胞生长.
- Caflc1和Shu1被确定为有效的异质血红素进口者,增强了外源血红素的生长.
- 过度表达Caflc1和Shu1导致猪肌内蛋白增加112.28%,大豆血红蛋白标位增加5.78倍.
结论:
- 提高血吸收效率是克服Pichia pastoris血缺乏症的可行策略.
- 确定的血红素进口商 (Caflc1和Shu1) 有效地提高了血红蛋白的生产.
- 这种方法为在Pichia pastoris中有效合成有价值的血蛋白提供了一个有希望的方法.
相关概念视频
Hemoglobin
3.1K
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
3.1K
Oxygen Transport in the Blood
2.3K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
2.3K
Erythropoiesis
4.0K
Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
4.0K
Factors Affecting Erythropoiesis
3.0K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
3.0K
Gene Families
8.7K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
8.7K
Lifecycle of Erythrocytes
1.7K
Erythrocytes, also known as red blood cells, constantly move through blood capillaries. As a result, they damage their plasma membrane due to the continuous friction. Typically, after 100 to 120 days, erythrocytes become rigid and fragile as they wear out. As they pass through small vessels in the spleen and liver, they can get trapped and break apart into fragments.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups....
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups....
1.7K

