Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.7K
Ribosome Profiling02:24

Ribosome Profiling

3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Large-Scale Dataset of Pre-Treatment TCR Repertoires in Colorectal Cancer Patients.

Scientific data·2025
Same author

The compositional behavior of the human T cell receptor repertoire in ovarian cancer compared to healthy donors.

Scientific data·2025
Same author

Self-supervised learning of T cell receptor sequences exposes core properties for T cell membership.

Science advances·2024
Same author

Ovarian cancer is detectable from peripheral blood using machine learning over T-cell receptor repertoires.

Briefings in bioinformatics·2024
Same author

The temporal behavior of the murine T cell receptor repertoire following Immunotherapy.

Scientific data·2023
Same author

Age-related loss of gene-to-gene transcriptional coordination among single cells.

Nature metabolism·2020

相关实验视频

Updated: May 15, 2025

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.1K

基于RNA-seq的T细胞谱系提取与TCR-seq相比较.

Linoy Menda Dabran1, Alona Zilberberg1, Sol Efroni1

  • 1The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel.

Oxford open immunology
|April 7, 2025
PubMed
概括

RNA测序很难准确地提取T细胞受体 (TCR) 序列,特别是互补性决定区域3 (CDR3) 变异. 对于可靠的曲目分析,TCR测序仍然是黄金标准.

科学领域:

  • 免疫学 免疫学 免疫学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 存在大量的RNA测序数据,为T细胞受体 (TCR) 谱系分析提供了潜力.
  • 目前从RNA测序数据中提取TCR序列的方法缺乏与既定黄金标准的验证.
  • 了解TCR曲目对免疫学和疾病研究至关重要.

研究的目的:

  • 评估从RNA测序数据中提取T细胞受体 (TCR) 序列的可行性和准确性.
  • 将RNA测序衍生的TCR数据与黄金标准TCR测序进行比较.
  • 评估测序对数据提取的读数长度和T细胞丰度对数据提取的影响.

主要方法:

  • 从RNA测序提取的TCR序列与TCR测序 (黄金标准) 的比较分析.
  • 评估使用75个基数对单端和150个基数对配对末端测序.
  • 计算T细胞丰度以评估与提取的TCR读数的相关性.

主要成果:

  • 从RNA测序和黄金标准TCR测序中获得的TCR序列之间观察到显著的差异.
  • 较长的测序读取长度并没有显著提高互补性确定区域3 (CDR3) 提取的准确性.
  • 从RNA测序中获得的T细胞丰度和TCR读数之间没有发现显著的相关性.
关键词:
在AIRR-seqq.在RNA-seqqq.提取T细胞谱的提取方法这就是TCR-seqq.

更多相关视频

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

9.4K
Simultaneous Quantification of T-Cell Receptor Excision Circles TRECs and K-Deleting Recombination Excision Circles KRECs by Real-time PCR
14:14

Simultaneous Quantification of T-Cell Receptor Excision Circles TRECs and K-Deleting Recombination Excision Circles KRECs by Real-time PCR

Published on: December 6, 2014

16.4K

相关实验视频

Last Updated: May 15, 2025

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.1K
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

9.4K
Simultaneous Quantification of T-Cell Receptor Excision Circles TRECs and K-Deleting Recombination Excision Circles KRECs by Real-time PCR
14:14

Simultaneous Quantification of T-Cell Receptor Excision Circles TRECs and K-Deleting Recombination Excision Circles KRECs by Real-time PCR

Published on: December 6, 2014

16.4K

结论:

  • 目前RNA测序不足以准确可靠地提取T细胞受体 (TCR) 序列.
  • 对于精确的曲目分析,TCR测序方法是必不可少的.
  • 研究人员应优先考虑TCR测序对于需要准确TCR数据的研究.