适应性进化和多重化工程Saccharomyces cerevisiae用于无中和剂生产的l-乳酸
Baoyuan Guo1,2,3, Wenwen Yu1,2, Xianhao Xu1,2
1Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Journal of agricultural and food chemistry
|April 7, 2025
概括
研究人员开发了一种新的酵母菌株,用于具有成本效益的,环保的l-乳酸生物生产. 这种改造的Saccharomyces cerevisiae突变体在没有中和剂的情况下实现了高产量,大大降低了成本和环境影响.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- l-乳酸是一种有价值的化学物质,具有广泛的应用.
- 目前的生物生产方法需要中和剂,增加成本和环境问题.
- 发展可持续和成本效益的乳酸生产至关重要.
研究的目的:
- 为了改造一种Saccharomyces cerevisiae突变体,以提高l-乳酸的生产.
- 在不需要中和剂的情况下实现高度的l-乳酸发酵.
- 建立一个环境友好的l-乳酸生物生产的基础.
主要方法:
- 使用适应性实验室进化来获得乳酸耐受性酵母突变 (TMG2).
- 应用了一种"推拉抑制"的代谢工程策略,以提高产量.
- 进行了运输蛋白pPfFNT的过度表达和NADH和乙-CoA供应的优化.
主要成果:
- 成功开发了一种能够耐受低pH环境 (pH2.60) 的突变菌株 (TMG2).
- 工程菌株TMG27的l-乳酸标位达到62.6g/L,改善了33.8%.
- 没有中和剂的发酵在pH值为2.90时产生了76.2g/L乳酸,创纪录的生产率为2.1g/Lh.
结论:
- 开发的酵母菌株和策略使得有效的l-乳酸生物生产成为可能.
- 消除中和剂可以显著降低生产成本和环境污染.
- 这项工作为可持续和工业规模的乳酸制造提供了一个强大的平台.
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