20纳米纳米粒子触发流入内皮细胞通过TRPV4通道
Jaspreet Singh Nagi1, Amber L Doiron1
1Department of Electrical and Biomedical Engineering, University of Vermont, Burlington, VT 05405, USA. amber.doiron@uvm.edu.
Biomaterials science
|April 7, 2025
概括
小金纳米粒子在剪切应力下增加内皮细胞中的细胞内,主要通过TRPV4通道. 这一发现对于理解纳米粒子毒性和改进输送技术至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 纳米技术纳米技术
背景情况:
- 细胞内的增加 (Ca2+) 是纳米颗粒对内皮细胞的已知影响.
- 精确的机制,特别是在剪切应力下,仍然不完全理解.
研究的目的:
- 为了研究20纳米粒子对切割应力下的内皮细胞中Ca2+水平的影响.
- 阐明在这个过程中临时受体潜在化物4型 (TRPV4) 通道的作用.
主要方法:
- 人类静脉内皮细胞 (HUVECs),人体冠状动脉内皮细胞 (HCAECs) 和人体心脏微血管内皮细胞 (HMVEC-Cs) 暴露于20纳米金粒子.
- 切割应力 (0.6Pa) 的应用和细胞内Ca2+水平的测量.
- 使用TRPV4通道抗剂来评估其对Ca2+流入的影响.
主要成果:
- 在所有测试的细胞类型中,在20nm黄金颗粒 (100μg mL-1) 中观察到细胞内Ca2+的显著增加,几乎是细胞内Ca2+的三倍.
- 在剪切应力下的HUVEC中,TRPV4通道对抗性降低了Ca2+水平的9.3倍.
- 与单独的流体流相比,暴露于20纳米粒子和流体流的内皮细胞表现出增强的对齐.
结论:
- 20纳米金颗粒激活TRPV4通道,导致细胞内Ca2+增加和潜在的内皮透性.
- 这些发现对于理解纳米粒子诱导的细胞效应和优化纳米粒子传递系统至关重要.
- 需要进一步的研究,以利用这种理解来实现更安全,更有效的纳米医学应用.
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