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用纳米孔序列数据识别病毒监测的生物信息管道:系统评估

Nick Laurenz Kaiser¹, Martin H Groschup¹, Balal Sadeghi¹

¹Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Greifswald, Insel Riems 17493, Germany.

Journal of virological methods

|April 7, 2025

概括

这项研究评估了病毒监测中纳米孔测序的生物信息学管道. 虽然两个管道是合适的,但需要集成的基调和用户友好的安装,以有效地进行病毒分子表征.

科学领域:

生物信息学是一种生物信息学.
病毒学病毒学
基因组监测基因组监测

背景情况:

纳米孔测序提供了便携式病毒监控功能.
处理长,易出错的纳米孔读取需要专门的生物信息学管道.
自动化管道对于向决策者提供及时的病毒分子数据至关重要.

研究的目的:

用纳米孔测序系统评估和比较用于病毒监测的生物信息学管道.
确定适用于有效的病毒基因组监测的管道.

主要方法:

系统的文献搜索确定了239篇最初的论文.
对22条选定的管道进行测试和功能范围比较.
将六条管道纳入最终评估,以合适性为基础.

主要成果:

两条管道被确定为适合纳米孔病毒监测的管道.
四条线下管道缺乏必要的下游分析步骤.
一个合适的管道仅在线并收费;另一个是免费和多功能 (在线/离线).
没有评估的管道整合了基调,也没有为所有分析提供单调解决方案.

结论:

现有的管道存在缺点,包括缺乏集成的基调和易于安装.
显然需要一个新的生物信息管道,集成基调并简化基于纳米孔的病毒监测分析过程.

关键词:

生物信息学是一种生物信息学.纳米孔测序的测序管道管道管道管道管道序列分析是指进行序列分析.第三代测序系统是第三代测序系统.病毒监测病毒监测

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