Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Southern Blot02:57

Southern Blot

18.0K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
18.0K
Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Predicting human age by detecting DNA methylation status in hair.

Electrophoresis·2021
Same author

Enzymatic in situ generation of covalently conjugated electron acceptor of PbSe quantum dots for high throughput and versatile photoelectrochemical bioanalysis.

Analytica chimica acta·2019
Same author

Epigenetic modifications but not genetic polymorphisms regulate KEAP1 expression in colorectal cancer.

Journal of cellular biochemistry·2019
Same author

Bisulfite-free direct detection of 5-methylcytosine and 5-hydroxymethylcytosine at base resolution.

Nature biotechnology·2019
Same author

Optimizing early enteral nutrition in severe stroke (OPENS): protocol for a multicentre randomized controlled trial.

BMC neurology·2019
Same author

A functional variant in the flanking region of pri-let-7f contributes to colorectal cancer risk in a Chinese population.

Journal of cellular physiology·2019

相关实验视频

Updated: May 15, 2025

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.0K

基于STR和生物流体特异性CpG标记物的编码检测系统的开发.

Zeqin Li1,2, Shiqi Liu1,3, Fang Yuan1,2

  • 1School of Forensic Medicine, Shanxi Medical University, Jinzhong, Shanxi, China.

Biotechnology and applied biochemistry
|April 8, 2025
PubMed
概括

法医科学家现在可以同时识别体液及其捐赠者,使用一种新的DNA分析系统. 这种综合方法通过结合甲基化标志物和短串重复 (STR) 分析来提高刑事调查中的证据有效性.

关键词:
身体液体识别 身体液体识别法医遗传学个人识别身份证甲基化敏感的限制酶-聚合酶链反应 (MSRE-PCR)短串联重复重复的重复.

更多相关视频

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.0K

相关实验视频

Last Updated: May 15, 2025

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.0K
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.0K

科学领域:

  • 法医科学 法医科学 法医科学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 准确识别体液及其捐赠者对于法医调查至关重要.
  • 现有的个人识别方法 (自体短串重复) 和体液来源 (DNA甲基化) 通常是单独执行的.
  • 需要一个集成的系统来同时进行子源和源级分析.

研究的目的:

  • 开发和验证一种代码检测系统,用于同时进行个人识别和生物流体原产地确定.
  • 将DNA甲基化标记物与用于毛细管电泳的短串重复 (STR) 放大系统集成.

主要方法:

  • 开发了一种多重系统,将7个复数DNA甲基化试验 (5个生物流体特异性CpG标记和2个对照) 与20个复数STR放大系统相结合.
  • 利用毛细管电泳来同时分析甲基化概况和STR类型.
  • 在单一来源和混合体液样本 (周围血液,精液,唾液,月经血液,阴道分泌物) 上测试了该系统.

主要成果:

  • 该系统成功地从单一来源区分了五种类型的体液.
  • 为含有不同比例的两到四种体液的混合样本生成DNA甲基化概况.
  • 实现了个人身份识别的高累积歧视能力 (1-3.5120 × 10-25).
  • 精液和阴道分泌物的有效识别证明了低DNA输入 (0.1 ng) 和长时间储存 (5 个月在室温下).

结论:

  • 开发的代码检测系统提供了一个强大的工具,用于在法医科学中同时识别体液和提供者概况.
  • 这种综合方法提高了刑事调查中证据分析的效率和有效性.
  • 该系统由于其灵敏度和稳定性,对常规法医应用具有前景.