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基于STR和生物流体特异性CpG标记物的编码检测系统的开发

Zeqin Li^1,2, Shiqi Liu^1,3, Fang Yuan^1,2

¹School of Forensic Medicine, Shanxi Medical University, Jinzhong, Shanxi, China.

Biotechnology and applied biochemistry

|April 8, 2025

概括

法医科学家现在可以同时识别体液及其捐赠者,使用一种新的DNA分析系统. 这种综合方法通过结合甲基化标志物和短串重复 (STR) 分析来提高刑事调查中的证据有效性.

科学领域:

法医科学法医科学法医科学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

准确识别体液及其捐赠者对于法医调查至关重要.
现有的个人识别方法 (自体短串重复) 和体液来源 (DNA甲基化) 通常是单独执行的.
需要一个集成的系统来同时进行子源和源级分析.

研究的目的:

开发和验证一种代码检测系统,用于同时进行个人识别和生物流体原产地确定.
将DNA甲基化标记物与用于毛细管电泳的短串重复 (STR) 放大系统集成.

主要方法:

开发了一种多重系统,将7个复数DNA甲基化试验 (5个生物流体特异性CpG标记和2个对照) 与20个复数STR放大系统相结合.
利用毛细管电泳来同时分析甲基化概况和STR类型.
在单一来源和混合体液样本 (周围血液,精液,唾液,月经血液,阴道分泌物) 上测试了该系统.

主要成果:

该系统成功地从单一来源区分了五种类型的体液.
为含有不同比例的两到四种体液的混合样本生成DNA甲基化概况.
实现了个人身份识别的高累积歧视能力 (1-3.5120 × 10-25).
精液和阴道分泌物的有效识别证明了低DNA输入 (0.1 ng) 和长时间储存 (5 个月在室温下).

结论:

开发的代码检测系统提供了一个强大的工具,用于在法医科学中同时识别体液和提供者概况.
这种综合方法提高了刑事调查中证据分析的效率和有效性.
该系统由于其灵敏度和稳定性,对常规法医应用具有前景.

关键词:

身体液体识别身体液体识别法医遗传学个人识别身份证甲基化敏感的限制酶-聚合酶链反应 (MSRE-PCR)短串联重复重复的重复.

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