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工程 Bacopa monnieri 为了提高酸盐含量及其神经学评估

Gajendra Singh Jeena¹, Sunil Kumar¹, Sachi Bharti^2,3

¹Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, CSIR-CIMAP) PO CIMAP, Near Kukrail Picnic Spot, Lucknow, 226015, India.

Applied microbiology and biotechnology

|April 8, 2025

概括

巴科帕蒙尼埃里的代谢工程增加了巴科赛德的产量. 增强的提取物改善了运动功能,并在帕金森病 (PD) 的大鼠模型中减少了多巴胺基耗尽.

科学领域:

植物生物技术植物生物技术
代谢工程是代谢工程.
神经药理学神经药理学

背景情况:

巴科西德,来自Bacopa monnieri的三基酸盐,具有显著的药理学益处,但受到了低可用性的困扰.
对于化物生物合成的特定代谢途径的了解仍然很少,这阻碍了提高生产的努力.
之前的研究还没有探索代谢工程策略,以增加Bacopa monnieri.中的化物含量.

研究的目的:

为了改造Bacopa monnieri以增强化物生物合成和含量.
在帕金森病 (PD) 鼠标模型中研究工程Bacopa monnieri提取物的治疗潜力.

主要方法:

过度表达Bacopa monnieri素合成酶 (BmSQS1-OE),以增加前体供应.
抑制 B. monnieri G10H (BmG10H-1-KD) 的作用,以将代谢流转向三烯生物合成.
在工程线路中绝对定量化物,并在6氧多巴胺 (6-OHDA) 诱导的PD小鼠模型中评估提取物的疗效.

主要成果:

经过工程改造的BmSQS1(OE) -BmG10H1(KD) 巴科帕蒙尼耶里菌株表现出明显改善的百科A3,百科II和百科A的含量.
工程植物提取物在改善PD大鼠的运动活动,神经肌肉协调和社交互动方面表现出增强的疗效.
免疫组织化学表明,增强的化提取物减少了大鼠大脑组织中6OHDA诱导的多巴胺基衰竭.

结论:

代谢工程策略,包括BmSQS1过度表达和BmG10H沉默,有效地增强了Bacopa monnieri.中的化物积累.
工程 Bacopa monnieri 提取物通过减轻多巴胺基神经退行,显示了神经系统疾病,特别是帕金森病的治疗潜力.

关键词:

巴科帕·蒙尼耶里 (Bacopa Monnieri) 是一个巴科西德是什么? 巴科西德是什么?过度表达是一种过度表达.它们是RNAiRNAi.类沙宁是一种类的沙素.三二烯是一种三二烯.

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