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将Dip-it-DART-Orbitrap-MS与等离子体对HPLC/Orbitrap-MS进行比较,以分析白葡萄中的芳香甘油结合

Mariusz Dziadas¹, Henryk Jeleń²

¹Faculty of Chemistry, University of Wrocław, Wrocław, Poland.

Journal of mass spectrometry : JMS

|April 8, 2025

概括

使用实时等离子体直接分析 (N2-DART) 和高分辨率质谱法 (Orbitrap) 的新方法可快速选葡萄中的芳香糖化物前体,显著缩短分析时间.

科学领域:

分析化学分析化学
植物生物化学植物生物化学
食品科学食品科学食品科学

背景情况:

在植物中直接分析芳香糖基前体具有挑战性.
传统的方法,如SPE-GC/MS,对完整的糖化物产生有限的信息.
高分辨率质谱和液体色谱学允许直接分析糖化物.

研究的目的:

开发一种新的,快速的方法来选白葡萄中的芳香糖基前体.
使用高分辨率质谱与实时直接分析 (DART) 软电离.
为了优化N2-DART参数来分析糖化前体.

主要方法:

高分辨率质谱仪 (Orbitrap) 与等离子体DART (N2-DART) 结合使用.
优化N2-DART电离参数 (电网电压,气体温度,采样器速度).
对15组合成甘氨酸前体进行选,并与HPLC/Orbitrap-MS进行比较.

主要成果:

使用N2-DART-Orbitrap-MS. 建立了用甘氨酸前体的特征性离子化模式.
证明了与已建立的HPLC/Orbitrap-MS方法可比的结果.
通过消除色谱分离,显著减少了分析时间.

结论:

N2-DART-Orbitrap-MS方法提供了一种快速有效的方法来选糖化前体.
这种技术允许使用等离子体进行更深层次的电离探索,这是一种用于糖化物分析的新型应用.
该方法适用于快速表征和选植物衍生糖结芳香化合物.

关键词:

在DART中使用DART.轨道上的陷宁电离的原因之一是宁电离.糖化物前体的前体.单烯是一种单烯.

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