在结核病治疗期间获得的连续采样的唾液样本中的污染率,以捕获培养转换
medRxiv : the preprint server for health sciences
|April 8, 2025
概括
监督的唾液收集显著减少了结核病培养物的污染. 没有监督的样本显示出更高的污染率,特别是在治疗后期,影响诊断准确度.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 临床诊断 临床诊断 临床诊断
背景情况:
- 唾液培养对于结核病 (TB) 诊断和治疗监测至关重要.
- MGIT液体培养物的微生物污染往往使它们无法解释.
- 以前的研究表明,冷链和监督收集可以减少污染,但纵向数据有限.
研究的目的:
- 评估处理时间和无监督采集与唾液培养污染率之间的关联.
- 为了估计结核病治疗12周的污染率.
- 确定污染的临床和行为预测因素.
主要方法:
- 在12周治疗期间,分析了301名结核病患者的3155个唾液样本.
- 每周采样和污染率的评估.
- 统计分析以确定污染的预测因素,包括收集监督和处理时间.
主要成果:
- 污染率从基线的12.3%上升到第8周的30%左右.
- 没有监督的样本采集与基线 (p=0.048) 和整体 (p=0.028) 较高的污染率显著相关.
- 在涂抹等级和污染风险之间观察到一个反向关系.
结论:
- 监督的唾液收集对于尽量减少结核病培养物的污染至关重要.
- 污染率在早期治疗期间增加,后来稳定,影响监测.
- 结果强调提高每周培养的实用性,特别是在基于社区的监测中.
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