对微生物病原体的监测和在干鱼片中开发黄金葡萄球菌的预测模型
Jeong A Han1,2, Sunyoung Hwang1, Nari Son1
1Division of Microbiology, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation, Cheongju, Chungbuk 28159 Republic of Korea.
Food science and biotechnology
|April 8, 2025
概括
这项研究评估了干鱼中的微生物污染,发现了低水平的金黄色葡萄球菌和透菌. 开发了S. aureus在干海鲜中的生存预测模型,显示了温度依赖的行为,以提高食品安全.
科学领域:
- 食品微生物学 食品微生物学
- 食品安全科学 食品安全科学
- 预测建模预测建模
背景情况:
- 干鱼产品容易受到微生物污染.
- 关键的食源性病原体对干燥海鲜消费者构成风险.
- 准确的微生物风险评估对于食品安全管理至关重要.
研究的目的:
- 为了评估干鱼片中的微生物污染.
- 为了确定特定的食物传播病原体的流行,如金黄色葡萄球菌.
- 开发各种海鲜干产品中S. aureus存活率的预测模型.
主要方法:
- 对580个干鱼片进行微生物监测.
- 用S. aureus对干鱼,Hwangtae-chae和调味干鱼进行注射.
- 在受控温度 (4°C,10°C,20°C,37°C) 的储存实验.
- 使用韦布尔分布和二次建模分析微生物生存数据.
主要成果:
- 没有沙门氏菌 spp.,Listeria monocytogenes,或菌 spp. 已经被检测到.
- 黄金葡萄球菌在一个样本中被发现;Clostridium perfringens在四个样本中.
- 黄金菌的生存率在干燥海鲜中表现出明显的温度依赖性行为.
- 开发的二次模型显示出良好的预测准确性 (RMSE 0.39-0.35).
结论:
- 干燥的鱼片显示了主要的食源性病原体的最小污染.
- 预测模型有效地预测S. aureus在不同温度条件下的存活率.
- 这些模型增强了对干燥海鲜产品的微生物风险评估和食品安全实践.
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