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Updated: May 21, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
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在MicroED中使用能量过恢复高分辨率信息

Max T B Clabbers1,2, Tamir Gonen1,2,3

  • 1Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095.

bioRxiv : the preprint server for biology
|April 8, 2025
PubMed
概括

能量过通过减少噪音和利峰值,显著提高了微晶电子衍射 (MicroED) 数据质量. 这种技术增强了高分辨率的结构信息,这对于宏分子晶体学至关重要.

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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