TENT5C延长了多A尾巴,以维持精子形态发生和生育能力
Marine Baptissart1, Ankit Gupta1, Alexander C Poirot1
1Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Durham, North Carolina 27709, USA.
bioRxiv : the preprint server for biology
|April 8, 2025
概括
聚甲尾聚合酶TENT5C对于精子形成至关重要. 它的缺失破坏了ODF1蛋白质的分布,导致无头精子和男性不孕.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 多基化是mRNA稳定性和翻译的关键.
- 特定的mRNA多样性 (A) 尾巴长度对于男性生育能力至关重要.
- 目前尚不清楚TENT5C在精子体转录基因组多体 (A) 尾部调节中的作用.
研究的目的:
- 为了研究TENT5C在调节精子类动物中多样性 (A) 尾巴长度方面的作用.
- 了解TENT5C活动如何影响ODF1蛋白位址和精子发育.
- 探讨多项A型尾巴造型与男性不孕症之间的联系.
主要方法:
- 在精子体转录组中分析了多样性 (A) 尾巴长度变化.
- 产生和分析与催化无活性TENT5C的小鼠.
- 评估精子形态和鞭毛结构.
主要成果:
- TENT5C活动对于维持ODF1转录的poly (A) 尾部长度至关重要.
- 丧失TENT5C功能导致精子体中ODF1蛋白分布受到干扰.
- 缺乏活性TENT5C的小鼠表现出严重的精子缺陷,包括无头精子和鞭毛异常.
结论:
- 在精子生成过程中,TENT5C多A) 聚合酶活性对ODF1mRNA的空间翻译至关重要.
- 由于TENT5C对poly(A) 尾巴长度的调节失调,可能导致男性不孕.
- 多尾形状分析是诊断导致不孕症的mRNA处理缺陷的宝贵工具.
相关概念视频
01:23Catenins
2.2K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.2K
01:13Five-Membered Heterocyclic Aromatic Compounds: Overview
3.6K
Heterocyclic aromatic compounds are cyclic compounds that are aromatic and have one or more heteroatoms—atoms other than carbon, in the ring. Depending upon the number of atoms present in the ring, they can be either five or six-membered. Examples of five-membered heterocyclic aromatic compounds include pyrrole, furan, thiophene, and imidazole. Pyrrole consists of one nitrogen atom having one lone pair of electrons. Furan and thiophene have one oxygen and one sulfur heteroatom,...
3.6K
01:26Tension Response at Adherens Junctions
2.6K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.6K
01:12Adaptability of Cytoskeletal Filaments
3.6K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.6K
01:18Assembly of Cytoskeletal Filaments
15.3K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
15.3K
02:26tRNA Activation
6.5K
6.5K