在四年内,循环的人类抗体中漂移的纵向分析
Collin Joyce1,2,3, Benjamin Nemoz1,2,4, Raiza Bastidas1,2,3
1Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA.
bioRxiv : the preprint server for biology
|April 8, 2025
概括
人类抗体库在四年内在规模和多样性方面基本保持稳定. 然而,特定的抗体基因组合发生变化,可能会影响对新威胁的免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 人类免疫系统依赖于多样化的抗体库,以有效地防御病原体.
- 据估计,循环抗体谱约为1015-1018个成员,对于识别和中和外来病原体至关重要.
- 通过V(D) J重组生成的抗体多样性表明了剧目组成的潜在时间波动.
研究的目的:
- 研究人类循环抗体库的时间稳定性.
- 为了确定四年时间内的抗体谱成分漂移的程度.
- 评估剧目中细微特征的变化如何影响免疫反应.
主要方法:
- 使用高通量测序来分析抗体库.
- 从前一项研究中,两名健康的捐赠者在大约四年后被重新分析.
- 分析的重点是V和J基因使用,CDRH3长度和特定的基因段组合.
主要成果:
- 抗体库的整体规模和多样性在四年内保持不变.
- 像V和J基因使用和CDRH3长度分布这样的总特征显示出稳定性.
- 在精细特征中观察到显著的变化,包括特定的V,J,D基因段组合和P/N核酸序列.
结论:
- 人类抗体库表现出其整体结构和大小随着时间的推移而显著的稳定性.
- 精细调整的曲目发生在特定的基因段组合和结节序列的改变.
- 这些精细特征的动态变化可能在适应免疫反应对新型病原体和疫苗方面发挥作用.
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