相关实验视频

Updated: May 15, 2025

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
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Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

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一个分裂的ALFA标签-纳米体系统用于蛋白质定位和近距离蛋白质在菌根菌中

Allison Fay1, Andrew P Kurland2,3, Zhuoning Li4

  • 1Immunology Program, Sloan Kettering Institute.

bioRxiv : the preprint server for biology
|April 8, 2025
PubMed
概括

一个新的ALFA标签-纳米体系统能够精确跟踪和操纵Mycobacterium结核病蛋白质. 这一突破有助于理解结核病生物学,并确定新的治疗点.

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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

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Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

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相关概念视频

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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