诱导癌细胞杀死使用DNA纳米结构介导死亡受体的超级集群
Göktuğ Aba1, Subinuer Abudukelimu2, Margot de Winter1
1Department of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZG Leiden, The Netherlands.
Nano letters
|April 8, 2025
概括
使用DNA纳米结构对瘤亡因子受体 (TNFR) 集群的精确控制可以增强癌细胞的杀死. 这种方法提高了对受体激活的理解,并为新的癌症疗法提供了信息.
科学领域:
- 生物化学和分子生物学
- 纳米技术在医学中的应用
- 癌症治疗方法 癌症治疗方法
背景情况:
- 第二类瘤亡因子受体 (TNFRs) 需要集群进行细胞内信号传输.
- 现有的TNFR聚类方法对联体价值和空间布局的控制有限.
- 低于最佳的TNFR激活可能会阻碍生物学洞察力和治疗疗效.
研究的目的:
- 开发一种使用DNA纳米结构精确控制TNFR聚类的方法.
- 为了研究连接体价值和空间组织对亡途径激活的影响.
- 探索工程结合体在增强癌症治疗中的潜力.
主要方法:
- 用工程单链TNF相关的亡诱导配体 (sc-TRAIL) 剪切器功能化DNA纳米结构的制造.
- 使用sc-TRAIL修剪器,将死亡受体5 (DR5) 与本地亲和力和几何结合起来.
- 根据不同的受体价值和分离,评估癌症有机体中的细胞杀死.
主要成果:
- 细胞杀死受到受体价值和受体间分离的显著影响.
- 在DNA纳米结构上的sc-TRAIL剪切剂的超集群增强了细胞死亡.
- 超集群sc-TRAIL剪切剂在诱导人类原发性胰腺和结肠直肠癌细胞杀伤中的已证明有效性.
结论:
- 通过DNA纳米结构对受体超级聚类的精确控制对于理解TNFR激活机制至关重要.
- 这种工程方法为开发更有效的癌症疗法提供了一个有希望的策略.
- 这些发现为设计优化食信号传递的向疗法提供了基础.
相关概念视频
01:17The Extrinsic Apoptotic Pathway
5.5K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
5.5K
02:26Protein Networks
3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
01:28Cells of the Innate Immune Response
1.1K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.1K
01:30Overview of Cell Death
5.6K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
5.6K
01:27Cytotoxic T Cells-mediated Immune Response
740
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
740
01:18Autophagic Cell Death
2.7K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
2.7K