芯片上的小肠显示在存在乳酸菌 (lacticaseibacillus rhamnosus) 生物膜的情况下产生生理性粘液分泌
Sanat Kumar Dash1,2,3, Cláudia N H Marques2,3, Gretchen J Mahler1,3
1Department of Biomedical Engineering, Binghamton University, Binghamton, New York, USA.
Biotechnology and bioengineering
|April 8, 2025
概括
研究人员使用肠道微生物群生物膜在芯片模型上开发了一种新的小肠. 这种先进的体外系统精确地模仿了人类的肠道环境,以改善药物发现和疾病研究.
科学领域:
- 胃肠道学和生物工程学
- 微流体学和芯片上器官技术
- 微生物学和免疫学
背景情况:
- 由于有限的可访问性,研究小肠是具有挑战性的.
- 目前的方法阻碍了对食物,药物,上皮和微生物群相互作用的研究.
- 需要新的微流体模型来复制肠道环境,用于研究和开发.
研究的目的:
- 开发一种微流体"芯片上的小肠"模型.
- 为了与 Lacticaseibacillus rhamnosus 生物膜共同培养肠细胞和杯细胞.
- 模仿人类小肠的生物和机械条件.
主要方法:
- 与L. rhamnosus生物膜一起培养的Caco-2和HT29-MTX细胞.
- 在低生理剪切应力 (0.3 mPa) 下进行动态培养,以促进生物膜的形成.
- 评估上皮质屏障的分化,极性,透性和粘素的产生.
主要成果:
- "芯片上的小肠"模型表现出差异化的上皮质屏障,具有类似人类的极性和透性.
- 在动态流量条件下,生物膜不会损害屏障的透性.
- 该模型在生物膜的存在下显示了增强的粘素分泌 (酸性,中性和MUC2).
- 动态模型保持了5天以上的功能,比静态模型 (1天) 长得多.
结论:
- 发达的"芯片上的小肠"提供了一个稳定的,在体内模仿的环境.
- 这种模型适用于研究肠道生理学,食物与肠道相互作用以及药物开发.
- 它为动物模型提供了一个有前途的替代方案,减少了对动物模型的依赖.
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