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Updated: May 15, 2025

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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使用基于板块的大小排除染色体和自动化进行高通量细胞外囊隔离

Tal Gilboa1,2, Dmitry Ter-Ovanesyan1, Clarissa May Babila1

  • 1Wyss Institute for Biologically Inspired Engineering, 201 Brookline Ave., Boston, Massachusetts 02215, United States.

Journal of the American Chemical Society
|April 8, 2025
PubMed
概括

我们开发了一种基于板块的自动化尺寸排除色谱 (SEC) 方法,用于从血中高通量隔离细胞外囊泡 (EV). 这种可扩展的平台可实现可复制的EV生物标志物发现和诊断.

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相关概念视频

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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